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Micro - Lab Practium > Practicum > Flashcards

Practicum Flashcards

1
Q

Fluid Thioglycollate Medium (O2, Aerotalerance)

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: N/A
Metabolic pathway: oxidation reduction
+ or – reaction:

(+) Indicator Resazurin turns pink when oxidized
Colorless die in reduced portion

Media and reagents used:

Media – basic and liquid medium designed to promote growth of wide variety of fastidious microbes: anaerobic and microaerophiles.

Takes O2 and reduces it to different molecule

Purpose for the test (why?)

Determine aerotolerance categories of three bacteria; typically anaerobic and microaerophilic. (Stratify)

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2
Q

Anaerobic Jar

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: N/A
Metabolic pathway: Conversion of H2 and O2 àWater
+ or – reaction:

Paper indicator confirms removal of oxygen (methylene blue when O2 present)
Growth will occur on plate inside jar is anaerobe or microaerophile

Media and reagents used:

Agar plates inoculated with bacteria
Sodium borohydride + sodium bicarbonate - CO2 Gas
Paladium catalyst = H2 and O2 Water

Purpose for the test (why?)

Remove oxygen to produce anaerobic environment for cultivation of anaerobes and microaerophiles

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3
Q

Motility: Hanging Drop technique

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: N/A
Metabolic pathway: N/A
+ or – reaction:

(+) Motility - Look for darting of cells
(-) Motility – Brownian motion created by collision with water molecules

Media and reagents used:

Water drop
Depression slide
Cover glass

Purpose for the test (why?)

Wet mount and hanging drop allows longer viewing of living cells to determine motility; Also used to view natural arrangement, shape, and size. (no heat fix or stain killing microbes)

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4
Q

Bile Esculin Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:
Metabolic pathway: Hydrolysis of esculin in bile
+ or – reaction:

(+) - If darkened in 48 hours = Id’ed as group D (streptococcus or enterococcus)
(-) – If darkened after 48 hours, not group D

Media and reagents used:

Medium – undefined, selective and differential;
Bile is the selective agent added
Ferric Citrate is added as a source of oxidized iron to indicate a positive test

Purpose for the test (why?)

Used commonly to presumptively ID streptococcus fecalis. Gram + testing.

Presumptive identification: An identification of a microorganism based upon simple colony and Gram stain observation and the results of relatively simple and inexpensive tests. Although a presumptive identification is believed to be valid, it may not be.

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5
Q

Mannitol Salts Agar

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: N/A Mannitol is a substrate for fermentation
Metabolic pathway: Fermentation (results in acid)
+ or – reaction:

(+) = yellow bacteria growth with halo; Staphylococcus aureus ; acidic
(-) = Growth appears pink or red and medium is unchanged; Staph, but not aureus
(--)= No growth, no color change; not staph

Media and reagents used

MEDIA:

Sodium Chloride (salt) makes the medium selective (for Staph) and differential (for Aureus). (MSA)

Bacteria are mostly water; causes dehydration.
Halophillic (salt loving) will grow in this medium

Contains pH sensitive dye and mannitol

Purpose for the test (why?)

Differentiate between specific strains of staphylococci
To identify S. Aureus

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6
Q

MacConkey Agar

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: N/A
Metabolic pathway: Fermentation (of lactose)
+ or – reaction:

(-) = colorless – basic; loses red color
(+)= Red color (acid presence) + growth = Fermentation + gram negative

(MacConkey’s sink is filthy. It smells like fermented lactose and he’s got an acidic attitude)

Media and reagents used:

Selective Media (allows gram-; inhibits gram+)

Contain pH indicators of fermentation (starts out pink)
Differential (between gram negatives) and selective: Bile salts and lactose, eliminating gram positive from thriving

Purpose for the test (why?):

Isolate and differentiate members of enterobacteriacae based on ability to ferment lactose.
Identify Gram negative.

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7
Q

Oxidation Fermentation Test (O-F Test)

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:
Metabolic pathway: Fermentation and Oxidation

Oxidation: ETC, oxygen final acceptor=CO2 waste
Fermentation: Acidic Waste

+ or – reaction:

(+) :

YELLOW (Sealed&UnSealed)– Fermentation and/or oxidation
Green/Blue – oxidation

Media and reagents used:

Medium – semi solid, low agar conc., allows determination of motility.
Mineral oil is used to seal out oxygen – oxidation should not occur

Purpose for the test (why?)

To differentiate bacteria on the basis of their ability to ferment or oxidize specific sugars.

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8
Q

Phenol Red Broth

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:
Metabolic pathway: (Glucose) Fermentation
+ or – reaction:

Yellow = (+) for glucose fermentation/Acidic
Pink = (-) for glucose fermentation
Bubble in Durham tube = positive for gas production.
Non turbid/No growth = inconclusive

Media and reagents used:

PR Broth (red broth):

differential test medium
Carbohydrate added
pH indicator (Phenol Red)

Inverted Durham tube tests for gas

Purpose for the test (why?)

Phenol Red (PR) Broth is used to differentiate members of enterobacteriaceae and to distinguish them from other gram-negative rods.
Test to see if bacteria can break down glucose through fermentation
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9
Q

Methyl Red (Components of IMVIC – to differentiate enterobacteria)

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:
Metabolic pathway: Mixed Acid FeRmentation
+ or – reaction:

(+) = Red at pH 4.4 (fermentation produced acid)
(-) = Yellow at 6.2 (closer to  basic)
Inconclusive = orange

Media and reagents used:

Combination Medium Broth (MR VP Broth)

Peptone+Glucose+Phosphate buffer
Methyl Red Indicator

Purpose for the test (why?)

designed to detect organisms capable of performing a mixed acid fermentation.
Differentiate between enterobacteriaceae and other gram neg rods

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10
Q

Voges-Praskauer Test (Components of IMVIC – to differentiate enterobacteria)

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:
Metabolic pathway: fermentation but quickly convert to neutral product acetoin.
+ or – reaction:

(+) = red (do not mistake copper color of reagent for red)
(-) = no color change

Media and reagents used:

MRVP BROTH
Reagent: VP Reagent A and B

Purpose for the test (why?)

designed for organisms that re able to ferment glucose, but convert their acid products to acetoin and 2,3-butanediol

Reagents added oxidize A&2,3B

Differentiate between enterobacteriaceae and other gram neg rods

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11
Q

Catalase Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:

Catalase: degrades H2O2 - H2O + O2 (g)
Superoxide Dismutasae: converts radicals into H2O2

Metabolic pathway: aerobic respiration
+ or – reaction:

(+) =  bubbles form (catalase present)
(-) = no bubbles form

Media and reagents used:

Hydrogen Peroxide

Purpose for the test (why?)

A. Test ability of microbe to degrade hydrogen peroxide
B. Identify organisms that produce catalase

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12
Q

Oxidase Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: cytochrome oxidase c (which accepts e- from phenylenediamine)
Metabolic pathway:

Oxidase removes an electron from Cytochrome C allowing electron acceptance of an electron from TMPD (pheylenediamine) reagent.

+ or – reaction:

(+) = dark blue within 20 seconds (caused by electron acceptance from phenylenediamine)
(-) = no color change within 20 seconds

Media and reagents used:

Reagent: reduced TMPD
Filter paper

Purpose for the test (why?)

Designed to identify the presence of cytochrome c oxidase

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13
Q

Nitrate Reduction Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: Nitrate Reductase
Metabolic pathway: Nitrate Reduction or denitrification
+ or – reaction:

A. Presence of Gas – (+) for denitrification / (+) Gas Bubbles (by known non-fermenter) = Denitrification

If not gas present must ADD reagents:

(+) = Red: Reduction of nitrate (NO3) to Nitrite (NO2)
(-) = no color; reduced to something else

Add zinc:

  • if turns red not reduced = no nitrate reduction (nitrate was left behind to react with zinc)
  • if no color change = Nitrate reduction**

Media and reagents used:

Nitrate Broth(undefined)
Durham Tube
Reagents:

Sulfanic Acid (NR A)
Naphthylamine (NR B)
Zinc

Purpose for the test (why?)

Check for ability to reduce nitrate (either to nitrite or denitrification) using enzyme nitrate reductase.

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14
Q

Citrate Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A
  • Enzyme**: Citrate-Permease*
  • Metabolic** _pathway_: Transport of molecules into a cell using citrate-permease .*
  • *+ or – reaction:**
(+) = growth or blue; citrate is utilized; basic/alkaline
(-) = no color change; no growth; neutral

Media and reagents used:

Simmon Citrate Agar

Contains sodium citrate as source for carbon
Ammonium phosphate for its nitrogen source.

pH Indicator

Brom thymol blue:

green at neutral pH
turns blue at alkaline (basic) pH >7.6.

Purpose for the test (why?)

Used to determine ability of organism to use citrate as a sole source of carbon.

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15
Q

Decarboxylation Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:

  • Decarboxylase* – removes carboxyl group (COOH); producing alkaline products (pH Test)
  • Deaminase* – removes amine group (NH2); uses reagent
  • *Metabolic pathway we are discussing** – fermentation; mineral oil used to seal out O2
  • *+ or – reaction:**

pH Indicator:

  • POSITIVE (+) = purple at 6.8* –alkaline products – decarboxylation took place
  • NEGATIVE (-) = yellow below 5.2*, still acidic, no carboxylation

Media and reagents used:

Decarboxylase moller
pH Indicator (purple)
Mineral Oil

Purpose for the test:

  1. Designed to differentiate members of enterobacteriaceae and to distinguish them from other gram-negative rods.
  2. Test for present of decarboxylase.
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16
Q

Phenylalanine Deaminase Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A
  • Enzyme**: *Phenylalanine Deaminase
  • Metabolic** _pathway_: *removes amine group to
  • *+ or – reaction:**
  • (+) = green; deaminase is present*
  • (-) = no color change or yellow is negative; deaminase is absent*

Media and reagents used:

  1. Phenylalanine Agar
  2. Ferric Chloride (FeCl3) - Reacts with phenylpyruvic acid

Purpose for the test (why?)

Test ability of organism to produce phenylalanine deaminase

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17
Q

Starch Hydrolysis

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A
  • Enzyme**: *a-amylase and oligo-1,6-glucosidase
  • Metabolic pathway:** *Hydrolysis of starch molecules

+ or – reaction:

(+) = clearing around growth; amylase present

Media and reagents used:

Starch Agar
Iodine used to reveal the clear areas.

Purpose for the test (why?)

Test ability of organism to hydrolyze starch

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18
Q

Urea Hydrolysis

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A
  • Enzyme**: *Urease
  • *Metabolic** pathway: Hydrolysis of urea into ammonia and carbon dioxide
  • *+ or – reaction:**

(+) = pink; urease present; alkaline

Partial pink means slow hydrolysis; urease present

(-) = orange or yellow; no urease present

Media and reagents used:

Urea Broth
Phenol Red as pH indicator

Purpose for the test (why?)

Ability of organism to degrade urea using urease

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19
Q

Casein Hydrolysis Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A
  • Enzyme**: *Casease
  • Metabolic** _pathway_: *Hydrolysis of casein (break down amino acids into nutrients)
  • *+ or – reaction:**
  • (+) = clearing in agar;* casease present
  • (-) = no clearing*; casease is not present

Media and reagents used:

Milk Agar – differential medium used to test for casease.

Purpose for the test (why?)

Determine is the organism can hydrolyze casein (protein in milk)

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20
Q

Gelatin Hydrolysis Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A
  • Enzyme:** *Gelatinase (exoenzyme)
  • Metabolic pathway:** *If gelatinase – can take up amino acid and use for metabolic purpose
  • *+ or – reaction:**
*(+) = Medium is liquified when gelatinase producing bacteria is present
(-) = Medium will not liquify*

Media and reagents used:

Nutrient Gelatin

Purpose for the test (why?)

Determine ability of microbe to produce gelatinase.

21
Q

DNA Hydrolysis Test

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: Deoxyribonuclease (DNase)
_Metabolic pathway:
+ or – reaction:_

(+) = clearing around organism; DNase present

Media and reagents used:

DNase Test Agar

Purpose for the test (why?)

  1. Test presence of DNase.
  2. Differentiate between organism that can produce DNase.
22
Q

SIM Medium

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

_Enzyme: _

SIM:

Cysteine Desulfurase (cysteine - pyruvate)
Thiosulfate Reductase (sulfur reduction to - sulfate)

INDOLE:

Tryptophanase

Motility

N/A

Metabolic pathway: Fermentation and/or Respiration

+ or – reaction:

SIM

(+) = blackening of medium means sulfur reduction
(-) = no blackening no sulfur reduction

INDOLE

(+) = Reagent caused red layer; Tryptophan broken down by tryptophanase
(-) = Reagent color unchanged

MOTILITY

(+) = Motile; Radiating growth from the culture stab line.

Media and reagents used:

  • BOTH use Combination Media* includes casein, animal tissue, amino acids
  • Indole test* uses Kovacs reagent which creates red layer when tryptophanase is present.

Purpose for the test (why?):

Tests for sulfur reduction, indole production using tryptophanase, and motility

23
Q

Blood Agar

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme:

Exotoxin-Hemolysins;
Streptolysins – if hemolysin from strep

S – Stable with O
O – Oh no, no O, anaerobe

  • *Metabolic pathway:** Destruction of RBCs
  • *+ or – reaction:**
  • (+) for B Hemolysis (BAD!) (Beta)* = Complete destruction of RBC; clearing of medium around colonies
  • (+) for A Hemolysis (alpha)*(Ay, he didda decent job) = partial destruction with green growth
  • (-) = y hemolysis (gamma)* (Y u no eat); simple growth, no media change.

Media and reagents used:

Blood agar = general purpose medium

Purpose for the test (why?):

Used to differentiate based on hemolytic characteristic

24
Q

Coagulase Tests

Enzyme:
Metabolic pathway:
+ or – reaction:
Media and reagents used:
Purpose for the test (why?)

A

Enzyme: Coagulase

  • Bound Coagulase:* also called clumping factor is BOUND TO THE BACTERIA
  • Free Coagulase* is extracellular
  • *Metabolic pathway:** Coagulase is attached to bacteria, reacts with fibrinogen, fibrinogen precipitates causing clumping
  • *+ or – reaction:**
  • (+) = Medium is solid;* plasma coagulated
  • (-) = Medium is liquid;* plasma did not coagulate

Media and reagents used:

Rabbit Plasma in a test tube

Purpose for the test (why?)

  1. Used to test presence of coagulase
  2. To differentiate between staph aureus and other gram+ bacteria
25
Q

What test does this image belong too?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/512/112/q_image_card.png?1417994698

A

Fluid Thioglycollate Medium (O2, Aerotalerance)

26
Q

What test does this image belong to?https://s3.amazonaws.com/brainscape-prod/system/cm/122/512/442/q_image_card.png?1417994754

A

Fluid Thioglycollate Medium (O2, Aerotalerance)

27
Q

This image is associated with what test?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/512/510/q_image_card.jpg?1417995032

A

Bile Esculin Test

28
Q

This image is associated with what test?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/513/285/q_image_card.jpg?1417995310

A

Mannitol Salts Agar

29
Q

This image is associated with what test?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/513/951/q_image_card.png?1417995407

A

Mannitol Salts Agar

30
Q

This image is associated with what test?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/514/116/q_image_card.jpg?1417995549

A

MacConkey Agar

31
Q

What test does this image belong too? What color is considered only oxidation? What color is considered fermentation and/or oxidation?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/514/320/q_image_thumb.png?1418016533

A

Oxidation Fermentation Test (O-F Test)

32
Q

What test is associated with this image?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/562/014/q_image_thumb.png?1418016806

A

Phenol Red Broth

Yellow = (+) for glucose fermentation/Acidic
Pink = (-) for glucose fermentation
Bubble in Durham tube = positive for gas production.
Non turbid/No growth = inconclusive

33
Q

What test does this image belong to?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/562/560/q_image_thumb.png?1418016966

A

Methyl Red

(+) = Red at pH 4.4 (fermentation produced acid)
(-) = Yellow at 6.2 (closer to basic)
Inconclusive = orange
34
Q

What test is this image assocaited with?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/562/646/q_image_thumb.png?1418017218

A

Voges-Praskauer Test

Key Info:

+ or – reaction:

(+) = red (do not mistake copper color of reagent for red)
(-) = no color change

Purpose for the test (why?)

designed for organisms that re able to ferment glucose, but convert their acid products to acetoin and 2,3-butanediol

Reagents added oxidize A&2,3B

Differentiate between enterobacteriaceae and other gram neg rodsVoges-Praskauer Test

35
Q

What test is associated with this image?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/563/047/q_image_thumb.png?1418017362

A

Oxidase Test

Key Info:

Enzyme: cytochrome oxidase c (which accepts e- from phenylenediamine)
Metabolic pathway:

Oxidase removes an electron from Cytochrome C allowing electron acceptance of an electron from TMPD (pheylenediamine) reagent.

+ or – reaction:

(+) = dark blue within 20 seconds (caused by electron acceptance from phenylenediamine)
(-) = no color change within 20 seconds
36
Q

What test does this image belong to?

A

Nitrate Reduction Test

Key Elements:

Enzyme: Nitrate Reductase
Metabolic pathway: Nitrate Reduction or denitrification
+ or – reaction:

Presence of Gas – (+) for denitrification

(+) Gas Bubbles (by known non-fermenter) = Denitrification

If not gas present must ADD reagents:

(+) = Red: Reduction of nitrate (NO3) to Nitrite (NO2)
(-) = no color; reduced to something else

Add zinc:

if turns red not reduced=no nitrate reduction (nitrate was left behind to react with zinc)
if no color change = Nitrate reduction

37
Q

What test does this image belong to?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/563/283/q_image_thumb.png?1418017593

A

Citrate Test

Component of IMVIC

Key Elements:

Enzyme: Citrate-Permease
Metabolic pathway: transport of molecules into a cell using citrate-permease .
+ or – reaction:

(+) = growth or blue; citrate is utilized; basic/alkaline
(-) = no color change; no growth; neutral

Media and reagents used:

Simmon Citrate Agar

Contains sodium citrate as source for carbon
Ammonium phosphate for its nitrogen source.

pH Indicator

Brom thymol blue:

green at neutral pH
turns blue at alkaline (basic) pH >7.6.

38
Q

This image is assocaited with what test?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/730/420/q_image_thumb.jpg?1418090792

A

Decarboxylation Test

Key Elements:

1. Enzyme:

a. Decarboxylase – removes carboxyl group (COOH); producing alkaline products (pH Test)
b. Deaminase – removes amine group (NH2); uses reagent

2. Metabolic pathway we are discussing – fermentation; mineral oil used to seal out O2

3. + or – reaction:

a. pH Indicator:

POSITIVE (+) = purple at 6.8 –alkaline products – decarboxylation took place

NEGATIVE (-) = yellow below 5.2, still acidic, no carboxylation

39
Q

This image is associated with what test?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/731/187/q_image_thumb.jpg?1418090973

A

Phyenylalanine Deaminase Test

Key Elements:

  1. Enzyme: Phenylalanine Deaminase
  2. Metabolic pathway: removes amine group to
    • or – reaction:
      a. (+) = green; deaminase is present
      b. (-) = no color change or yellow is negative; deaminase is absent
40
Q

What test does this image represent?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/731/994/q_image_thumb.jpg?1418091272

A

Starch Hydrolysis

Key Elements

  1. Enzyme: a-amylase and oligo-1,6-glucosidase
  2. Metabolic pathway: Hydrolysis of starch molecules
    • or – reaction:
      a. (+) = clearing around growth; amylase present
  4. Iodine used to reveal the clear areas
41
Q

What test is this image associated with?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/781/790/q_image_thumb.jpg?1418108531

A

Urea Hydrolysis

Key Elements:

  1. Enzyme: Urease
  2. Metabolic pathway: Hydrolysis of urea into ammonia and carbon dioxide
    • or – reaction:
      a. (+) = pink; urease present; alkaline
      i. Partial pink means slow hydrolysis; urease present
      b. (-) = orange or yellow; no urease present
  4. Media and reagents used:
    a. Urea Broth
    b. Phenol Red as pH indicator
42
Q

This image is associated with what test?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/782/201/q_image_thumb.jpg?1418108802

A

Casein Hydrolysis Tests

Key Elements

  1. Enzyme: Casease
  2. Metabolic pathway: Hydrolysis of casein (break down amino acids into nutrients)
    • or – reaction:
      a. (+) = clearing in agar; casease present
      b. (-) = no clearing; casease is not present
  4. Media and reagents used:
    a. Milk Agar – differential medium used to test for casease.
43
Q

What Test is this image associated with?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/782/298/q_image_thumb.jpg?1418108997

A

Gelatin Hydrolysis Test

Key Elements:

  1. Enzyme: Gelatinase (exoenzyme)
  2. Metabolic pathway: If gelatinase – can take up amino acid and use for metabolic purpose
    • or – reaction:
      a. (+) = Medium is liquified when gelatinase producing bacteria is present
      b. (-) = Medium will not liquify
44
Q

What test is this image associated with?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/782/451/q_image_thumb.jpg?1418109141

A

DNA Hydrolysis Test

Key Elements:

  1. Enzyme: Deoxyribonuclease (DNase)
  2. Metabolic pathway: N/A
    • or – reaction:
      a. (+) = clearing around organism; DNase present
  4. Media and reagents used:
    a. DNase Test Agar
45
Q

What test is associated with this image? What do the different “letters” mean?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/783/280/q_image_thumb.jpg?1418109659

A

Blood Agar

Key elements:

a. Exotoxin-Hemolysins;
b. Streptolysins – if hemolysin from strep
i. S – Stable with O
ii. O – Oh no, no O, anaerobe
2. Metabolic pathway: Destruction of RBCs
3. + or – reaction:
a. (+) for B Hemolysis (BAD!) (Beta) = Complete destruction of RBC; clearing of medium around colonies
b. (+) for A Hemolysis (alpha)(Ay, he didda decent job) = partial destruction with green growth
c. (-) = y hemolysis (gamma) (Y u no eat); simple growth, no media change.

46
Q

These images are associated with what series of tests?

https://s3.amazonaws.com/brainscape-prod/system/cm/122/783/447/q_image_thumb.jpg?1418109844

A

IMVIC Results

47
Q

IMViC stands for what?

A

Except for the lowercase “i”, which is added for ease of pronunciation, each of the letters in “IMViC” stands for one of these tests:

“I” is for indole test;

“M” is for methyl red test;

“V” is for Voges-Proskauer test, and

“C” is for citrate test. The lower case “i” is merely for “in”, as the Citrate test requires coliform samples to be placed “in Citrate”.

48
Q

The ____ ____ are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. A coliform is a gram negative, aerobic or facultative anaerobic rod which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination.

A

IMViC Tests

49
Q
A

Micro - Lab Practium (1 decks)

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