Practicals Flashcards
1
Q
What mutation cause sickle cell haemoglobin?
A
HbS has single mutation on aa 6 substituting charged glutamate to uncharged valine
2
Q
What is Vmax?
A
Maximum enzyme velocity - approached aymptotically
3
Q
What is Km?
A
The concentration at which the rate is exactly half Vmax
4
Q
What is V0?
A
Initial velocity - calculated by beer-lambert law
5
Q
What is a lineweaver-burk plot?
A
1/V0 against 1/[S]
6
Q
How do work out the y-intercept of a Lineweaver-burk plot?
A
1/Vmax
7
Q
How do you work out the x-intercept of a lineweaver-burk plot?
A
-1/Km
