Practicals

Question 1

What solution was used to lyse cells at the beginning of the cheek cell practical?

Answer 1

Cell Lysis Solution

Question 2

What solution is added straight after cheek cells were lysed?

Answer 2

Protein Precipitation Solution

Question 3

What two ingredients were used for DNA precipitation in the cheek cell practical?

Answer 3

Isopropanol and glycogen.

Question 4

If DNA from a female was electrophoresed in the cheek cell prac, what size and number of bands would we see?

Answer 4

One band around 490 bp.

Question 5

If DNA from a male was electrophoresed in the cheek cell prac, what size and number of bands would we see?

Answer 5

Two bands around 490 and 340 bp.

Question 6

How do dyes illuminate DNA in gel?

Answer 6

They intercalate between the stacked bases and produce fluorescence under UV light.

Question 7

Why does RNA separate less in gel electrophoresis?

Answer 7

It is smaller and single-stranded.