Practical Skills

Question 1

Describe how you would ensure that all the sodium hydroxide was transferred to the volumetric flask.

Answer 1

washing the container with deionised water where NaOH was dissolved, as well as the glass rod and the funnel and the filter.
Washings should be added to the volumetric flask

Question 2

Explain why lysine leaves the chromatography column after alanine.

Answer 2

alanine has greater attraction for the
stationary phase. greater affinity for stationary phase

Question 3

purpose of hydrogen carbonate

Answer 3

to neutralise the acid / H+
(1)
* so that the reactions is quenched / stopped