Practical Review

Question 1

Penicillin/Streptomycin

Answer 1

Antibiotics to prevent cell culture contamination

Question 2

2-Mercaptoethanol

Answer 2

Reducing agent used to prevent toxic levels of oxygen free radicals, particularly for immune cell types which are proliferating rapidly or secreting products

Question 3

10% Foetal Calf Serum

Answer 3

Provides growth factors and nutrients to facilitate cell survival and proliferation

Question 4

Polymixin B

Answer 4

Binds any endotoxins, such as LPS, in the media to prevent early maturation

Question 5

How does a DC Recognise the Yeast as a Pathogen?

Answer 5

via Pathogen-Associated Molecular Patterns (PAMPs)
However, in the practical we used bead particles - which would be recognised by Damage Associated Molecular Patterns (DAMPs)

Question 6

What is Diff Quick?

Answer 6

Used as a cytoplasmic stain to see cytoplasmic detail, such as intracellular uptake of pathogenic material

Question 7

Why is Detection of IgM Antibodies Early in COVID Infection Problematic?

Answer 7

Due to cross-reactions resulting in false-positive results

Question 8

When will Patients make Antibodies to COVID?

Answer 8

7-9 days for IgM
11-12 days for IgG