PRACTICAL C: Cell biology

Question 1

Average size of Zea mays cell

Answer 1

Small: 5 microns

Large: 220 microns

Question 2

Size of free living protozoan Amoeba

Answer 2

120*90 microns

Question 3

Size of cell in epidermis

Answer 3

8*3 microns

Question 4

Mean size of RBC

Answer 4

6.8 microns

Question 5

Mean size of WBC

Answer 5

10.3 microns

Question 6

Why is there a wider range of sizes for WBC than RBC?

Answer 6

More diversity of cell types in WBC

Question 7

Bacteriococcal cell size

Answer 7

0.6 microns

Question 8

Pink cells are Gram__

Answer 8

gram -ve

Question 9

Violet cells are Gram___

Answer 9

Gram +ve

Question 10

How long is the scale on the calibration slide?

Answer 10

1mm (100*10 microns)

Question 11

What measure is taken during the calibration to reduce experimental error?

Answer 11

Using the entire length of the shorter scale

Question 12

What do motor proteins do?

Answer 12

They generate cell motility, as they are ATPases and convert the chemical energy in ATP into mechanical work.

Question 13

How does myosin generate movement?

Answer 13

By its interaction with actin

Question 14

How do kinesin and dynein generate movement?

Answer 14

By their interactions with microtubules

Question 15

Paramecium motility

Answer 15

It’s a ciliate, uses microtubule-based motility to swim (ciliary motion)

Question 16

Amoeba motility

Answer 16

Uses amoeboid movement, generated by actin and myosin to crawl along surfaces (cytoplasmic projections generated by cytoskeleton).

Same type of movement found in WBC

Question 17

What are the dimension of the central square of the haemocytometer?

Answer 17

1mm²

Question 18

How is speed determined?

Answer 18

Distance/time

Question 19

How is speed determined in body length/s?

Answer 19

speed/body length

Question 20

Time taken to cover a body length

Answer 20

body length/speed