Practical 4 - Enzyme Kinetics

Question 1

What artificial enzyme substrate is used for chymotrypsinogen?

Answer 1

GPNA.

GPNA hydrolysed forming N-G-L-P and p-nitroaniline.

Question 2

At what wavelength does p-nitroaniline have a significant absorption?

Answer 2

410nm

Question 3

What is the rate of enzyme catalysis?

Answer 3

Vo=Vmax x (S)/((S) + Km)

Vmax = max enzyme velocity

Km = substrate conc at which RoR is exactly half of that of the Vmax