Practical Flashcards

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1
Q

Why do we grind the sample in a mortar and pestle?

A

This breaks down the cell wall

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2
Q

Why do we mix the sample with the detergents?

A
  • This breaks the cell membrane, releasing the cell contents into solutions
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3
Q

Why do we add salt to the sample?

A

This breaks the hydrogen bonds between the DNA and the water molecules

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4
Q

Why so we add a layer of ethanol on top of the sample?

A

alcohol causes the DNA to precipitate out of the solution

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