Practical Flashcards
1
Q
Why do we grind the sample in a mortar and pestle?
A
This breaks down the cell wall
2
Q
Why do we mix the sample with the detergents?
A
- This breaks the cell membrane, releasing the cell contents into solutions
3
Q
Why do we add salt to the sample?
A
This breaks the hydrogen bonds between the DNA and the water molecules
4
Q
Why so we add a layer of ethanol on top of the sample?
A
alcohol causes the DNA to precipitate out of the solution