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Microbiology. INFECTION > Practical > Flashcards

Practical Flashcards

1
Q

Laboratory diagnosis of infectious diseases are 2 methods, name them

A

Direct methods
Indirect methods

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2
Q

Direct methods are

A

Detection of pathogen

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3
Q

Indirect methods are

A

Detection of Abs in patient serum
اخذناها في الاميون

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4
Q

Obtain sample (specimen) according to patient ____

A

complaint
مثل البلغم

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5
Q

Direct methods are 3 types , name them

A

Microscopy

Non-culture methods

Isolation on culture medium tion of colony

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6
Q

In the microscopy method , direct film detect ______

A

Morphology of bacteria

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7
Q

Non-culture methods are 2 ways

A

Nucleic acid detection(e.g pcr)

Ag detection(direct immunofluorescentence)

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8
Q

Isolation on culture medium are 2 steps ,first _____ with its 4 ways
Then ________

A

Identification of colony

Antibiotic susceptibility tests

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9
Q

Identification of colony by

A

Colonial morphology
Microscopic appearance
Motility tests
Biochemical reactions

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10
Q

What is culture media?

A

Artificial substances → Supply bacteria with nutrients → Growth & multiplication

Visible effect

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11
Q

What are Visible effect of bacteria

A

Visible effect
Colony : on solid media
Turbidity: on fluid media

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12
Q

Types of media

A

Enriched
Basic
Selective
Enrichment
Media for anaerobic culture

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13
Q

BASIC MEDIA Constituents

A

Contain minimal nutrients → Growth of bacteria that don’t have special nutritional requirements

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14
Q

Examples for basic media :

A

Nutrient broth
Peptone H20
Nutrient agar

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15
Q

Nutrients broth are

A

Peptone H20 + Meat

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16
Q

Nutrient agar contain

A

Nutrient broth + agar powder

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17
Q

ENRICHED MEDIA
Constituents and Uses–>

A

Contains blood, serum or egg—>
Growth of fastidious organisms (need ↑ nutrients) which can’t grow on basic media

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18
Q

E.g for ENRICHED MEDIA

A

Blood agar
Chocolate agar
Loeffler’s serum

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19
Q

Blood agar is

A

Nutrient agar + blood

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20
Q

Chocolate agar is ______ which
Release _____

A

Heated blood agar

Factor X and V

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21
Q

Loeffler’s serum
Contain

A

Horse serum + glucose broth

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22
Q

Bacteria in Blood agar differentiated according to their _____

A

haemolytic activity

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23
Q

Hemolytic activity of bacteria are

A

Alpha (partial ) haemolysis
Gama(No ) haemolysis
Beta(complete) haemolysis

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24
Q

Selective media contains

A

Contains selective substance: chemicals or antibiotics

25
Selective media uses
Inhibits growth of some organisms (mainly flora or commensals) → allows growth of others → isolation of organisms from specimens containing flora e.g stools,throat swab
26
Types of selective media
Selective and enriched Selective " and differential
27
selective & Enriched Are
Thayer Martin Lowenstein Jensen(green fluid) Blood tellurite احفظ شكلهم
28
Thayer Martin contain
Chocolate agar + Antibiotics
29
Lowenstein Jensen contain
Egg + Malachite green
30
Blood tellurite contain
Blood agar + K tellurite
31
E.g for selective media selective
Sabouraud Dextrose agar Dextrose سكر العنب
32
Name Culture of fungi agar
Sabouraud Dextrose agar
33
In selective media selective: •______ →- bacteria •______→ - saprophytic fungi
•Chloramphenicol + Acidic pH Cyeloheximide
34
Selective & Differential Constituents :
Contains selective substance + sugar & indicator (changes in color if pH changes)
35
Selective & Differential Uses :
If organism ferments sugar → acidic pH → indicator changes in color
36
E.g for selective and Differential media
1-MacConkey 's agar 2-Thiosulfate citrate bile sucrose agar(TCBS) 3-Deoxycholate Citrate agar (DCA) احفظ أشكالهم
37
Selective substance of macConkeys agar
1) Bile salts 2) Crystal violet
38
Selective substance of TCBS
1) Bile salts 2) Thiosulfate + citrate 3) Alkaline pH
39
MacConkeys are used on with type of bacteria
Culture of intestinal Gram -ve bacilli
40
Thiosulfate citrate bile sucrose agar (TCBS) are used on which type of bacteria
Culture of Vibrio
41
Suger and indicator of macConkeys is
Lactose & Neutral red (pink transparent)
42
Suger and indicator of TCBS
Succrose and Bromothymol blue (Green)
43
Non Lactose fermenter ---> MacConkeys agar is
Pale yellow colonies
44
Lactose fermenter--->MacConkeys agar is
Rose pink colonies
45
Non Succrose fermenter--->TCBS agar is
No reaction
46
Succrose fermenter---->tcbs agar is
Yellow colonies احفظ الشكل
47
ENRICHMENT MEDIA Constituents
Fluid Selective → Inhibit growth of intestinal commensals (flora)
48
ENRICHMENT MEDIA Uses
→ Preliminary step for isolation of intestinal pathogens
49
ENRICHMENT MEDIA e.g
Selenite broth Tetrathionate broth Alkaline peptone H2o لا شكل
50
CULTURE MEDIA FOR ANAEROBE steps
A) 1st on Robertson cooked meat---> Broth + cooked meat (contains hemin & glutathioneتسحب الاوكسجين)----> Subculture on solid medium ( Nutrient agar Or Blood agar) ----> Anaerobic environment----> B) Anaerobic GasPak System
51
BIOCHEMICAL REACTIONS Methods are
Conventional Methods Commercial Modifications of Traditional Biochemical tests
52
I-Conventional Methods are
Effect on proteins Ability to utilize citrate Effect on proteins &Carbohydrates Enzymatic tests
53
Enzyme tests are
Catalase test Coagulase test Oxidase test Gelatinase test Urease test
54
Effect on proteins are
Indole test Urease test Gelatinase test
55
Ability to utilize citrate E.g
Citrate test
56
Effect on proteins & Carbohydrates E.g
Litmus milk Triple sugar iron (TSI) agar
57
Catalase test mechanism
Colony + H2O2--->Catalase---> H2O+O2 (Effervescence) احفظ الصورة
58
Oxidase test mechanism
Reagent (colorless) turns purple
59
Gelatinase test mechanism
Breaking down gelatin (semisolid< 24 C) Irreversible liquefaction تذكر الصورة

Microbiology. INFECTION (4 decks)

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