Pq Flashcards
Orphan viruses -
do not cause illnesses
What is the name of the lesion seen on the choric-allantoic membrane of an embryonated turkey egg, infected with proxyvirus?
Pock
The nutritional component of calf foetus:
Protein source and mediators for cell division from colostrum free calves
Affinity chromatography viral purification method:
virus specific antibodies bound to chromatograpy matrix. Adsorption of viruses. Elution with buffer.
Virus capsid surface proteins:
Capsomers
Real time polymerase chain-reaction:
Fluorescent labeling, detection of amplified products. Quantification. Computer analysis.
Polyacrylamide gel electrophoresis determines:
the virus amount and size (molecular weight) . Continuous NA thread or segmented.
Cells attached to viruses: I had a stroke trying to read this Q – Don’t know if it’s correct
haemagglutinate, cause haemagglutination inhibition, cause haemadsoprtion, directly spread from one cell to another by cell fusion
RNA-dependent RNA-polymerase enzyme found:
-/+ ss RNA plus dsRNA
Mutations: can happen during viral eclipse
- spontaneous/induced – Idk the answer can’t find anything
Offspring viruses cannot form in the infected cells of:
latent infections
During tolerated infections:
there is no immune response against the virus
Indirect virus detection method:
ELISA, Viruse Neutralisation, HAI, plaque reduction?
The haemagglutination inhibiting titer:
the highest (serum) dilution where we do not see haemaggluttination
A changing viruses’ continuous whey:
two serial tenfold dilution of virus suspension adding neg/pos serum.
Oseltamivir and zanamivir block
Tamiflu and Zanamivir neuraminidase inhibiotrs against influenza.
In young animals, the mothers antibodies: - Cant Find Answer
block the active immunodeficiency of the vaccine
Experimental animals can be used to spread viruses for diagnostic purposes? vaccine production vaccine control/checks cell line production
Yes - diagnostic purposes
Yes - vaccine production
Yes - vaccine control/checks
No - cell line production
With Cell culture passage ? we can keep up/maintain breeding we can increase the quantity of cell breeding/cultures we can remove contaminated viruses we can remove tumour cells
Yes - we can keep up/maintain breeding
Yes - we can increase the quantity of cell breeding/cultures
No - we can remove contaminated viruses
No - we can remove tumour cells
- The polymerase chain-reaction contains
sample (virus) DNA
temperature resistant (Taq) DNA polymerase
virus-specific oligonucleotide primers
virus-specific monoclonal antibodies
Yes - sample (virus) DNA
Yes - temperature resistant (Taq) DNA polymerase
Yes - virus-specific oligonucleotide primers
No - virus-specific monoclonal antibodies
Disinfectants always damage the viral nucleic acid only act against enveloped viruses can be used in the environment or outer/external cover occasionally toxic or corrosive
No - always damage the viral nucleic acid
No - only act against enveloped viruses
Yes - can be used in the environment or outer/external cover
Yes - occasionally toxic or corrosive
- acridine orange turns the single-stranded nucleic acid orange/red
- with polymerase chain-reaction we can determine the infective titer of the virus
- with the virus neutralisation test we can separate the maternal and vaccine-induced antibodies
- with the haemagglutination inhibition test, we can show the African swine fever antibodies
Yes - acridine orange turns the single-stranded nucleic acid orange/red
No - with polymerase chain-reaction we can determine the infective titer of the virus
No - with the virus neutralisation test we can separate the maternal and vaccine-induced
antibodies
No - with the haemagglutination inhibition test, we can show the African swine fever antibodies
What can we call the process where the cell division of cells of ectopic tissues are inhibited during contact?
contact inhibition
What is the concentration mode in which, with the help of osmotic pressure, water is removed from the virus suspension?
dialysis
What do we call the virus penetration method, whereby RNA-protein complexes can pass through the cytoplasmic membrane?
translocation
What is it called, when an mRNA codes for more than one protein?
Polycystronic
What do we call the type of phenotypic mix, when one of the virus’ nucleic acids builds into the other virus’ capsid?
I think its called ‘Transcapsidation’ Phenotypic mixing (melange
What do we call virus transfer via sexual contact?
Venereal infection
Indirect virus propagation disease in which you have to take two samples:
1st at onset of symptoms
2nd at late stage 12-14 days after
What is the name of the method whereby, organ samples of dead animals are inoculated for cell cultures?
Adsorption? Isolation? Cell cultivation or cell culture contamination
What is the name of the virus detection method whereby, the antigen and antibody react with one another via an electric current?
cc immunoelectrophoresis ccIEF
What do we call an the ingredients of an inactive vaccine, which increases the vaccine intensity?
Adjuvant
Papilloma viruses:
are usually stenoxen viruses
Alpha herpes viruses:
cause latency in ganglionic nerve cells
Orthopox viruses
causes cowpox virus
Beak and feather disease viruses:
have an immunosuppressive effect
Orthoreo viruses:
cause tenosynovitis in birds
Examples of the Picornaviridae family:
entero, tescho, gelato, tremo, cardio and alpha virus
Alpha viruses:
among them are zoonotic agents
A member of the Flavivirus is:
the diarrhoea virus in cattle/West Nile
Toroviruses:
in foals causes stomach inflammation and enteritis also calves
Some animal species are responsive to:
Nipah virus
The Ebola virus:
can cause bleeding fever in humans
The environmental owners/maintainers of the mamaerena viruses are
Rodents
Viruses affecting humans and cattle:
Deltaretrovirus genus
Bacteriophages:
can be used up for some bacteriological diagnostic tests
Prion proteins:
are usually spread orally
In chickens, it can cause kidney failure:
Gammacoronavirus (infectious bronchitis)
In horses, it causes a slowly-developing infection:
infective anaemia
Can cause immunosuppression in cats
- Panleicopenia virus
- peritonitis virus infecting cats
- feline leukaemia virus
- the infectious bursitis virus
No - Panleicopenia virus
Yes - peritonitis virus infecting cats
Yes - feline leukaemia virus
No - the infectious bursitis virus
- Newcastle disease virus
- causes human rubeola (rubulavirus)
- sheep adenocarcinoma virus adenovirus
- diarrhoea-causing enterovirus in cattle
No - Newcastle disease virus
No - causes human rubeola (rubulavirus)
No - sheep adenocarcinoma virus adenovirus
Yes - diarrhoea-causing enterovirus in cattle
Causes abortion, respiratory and central nervous system symptoms
- herpresmamillitis virus (BoHV-2)
- cattle affecting infectious rhinotracheitis virus (BoHV-2)
- equine rhinopneumonitis virus (EHV-1)
- Aujeszky-disease virus (SuHV-1)
No - herpresmamillitis virus (BoHV-2)
Yes - cattle affecting infectious rhinotracheitis virus (BoHV-2)
Yes - equine rhinopneumonitis virus (EHV-1)
Yes - Aujeszky-disease virus (SuHV-1)
Small mammals are the hosts of….
- mammalian bornavirus
- mammaerenavirus
- mamastroviruses
- hantaviruses
Yes - mammalian bornavirus
Yes - mammaerenavirus
No - mamastroviruses
Yes - hantaviruses
What is the name of the adeno- or orthoreovirus disease affecting calves?
Pneumoenteritis
What illnesses does the Aujeszky-disease virus cause in dogs?
Neural symptoms
In what animal, does this virus cause mouth pain?
Sheep
What is the name of the disease caused by goose parvovirus?
Derzsy disease
What is the name of the illness/disease caused by rhinovirus?
Rhinitis
In what species does Border disease virus cause illness?
Sheep
Which virus causes persistent infections in the sexual organs/tracts?
Arterovirus
Which animal virus did they manage to get rid of on Earth?
Rinderpest
. Which virus causes glandular stomach disease in parrots?
bornavirus
What do they call the virus causing human immunodeficiency?
AIDS
Polyomavirus causes latent infections.
TRUE
Adenovirus causes enteritis in mammals/birds
FALSE (Does cause pneumoenteritis)
Describe the morphology of the Adenovirus.
Linear dsDNA virus with an icosahedral capsid, nonenveloped and replicates in the nucleus.
What type of virus is Varicellovirus?
Herpesvirus (alphaherpesvirus)
Pox Virus causes skin lesions.
TRUE
Capripox causes skin lesions
TRUE
What type of virus is Hepadnaviridae?
Circular ss/ds DNA enveloped
Describe the morphology of Circoviridae
Circular ssDNA virus. Non enveloped. Icosahedral capsid
Parvo causes enteritis.
TRUE
How is the transmission of Birnaviridae?
By contact. – true but not in lectures. What is of note is that it has to be propagated in species specific tissues – difficult.
Rotavirus causes neonatal enteritis.
TRUE
What is the host of Teschovirus?
Pigs
What does Parechovirus cause?
Enteritis
Avastrovirus causes what in chickens?
Nephritis
Dicistroviridae is a virus of honey bees.
TRUE
How is Rabbit Haemorrhagic Disease transmitted?
By contact (direct or by fomites) True but not in lectures. What is of note is that RHD can not be propagated in vitro.
Border Disease causes abortion in sheep.
TRUE
Arteriviridae affects stallions.
TRUE (Can cause lifelong infection, but not necessarily a decrease of fertility)
The Influenza host (avian).
Wild water birds – don’t know. Not in lectures.
The surface protein of Influenza?
hemagglutinin (HA), neuraminidase (NA), matrix (M2
Parainfluenza virus 3 infects?
Cattle and Sheep – “Shipping Fever”
What type of virus is Canine Distemper Virus?
Morbillivirus (Paramyxoviridae)
Cowpox/pseudopox is zoonotic
TRUE
Herpes virus is an arbovirus (transmitted by insects).
FALSE
What does Herpesvirus cause?
Lesions (usually oral or genital)
How is Ephemerovirus transmitted?
By mosquitoes (arthropod bites)
Nairovirus is an arthropod virus.
TRUE (ticks)
What lesions does a Retrovirus cause?
Lesions in skin and brain (carcoma, carcinoma, leukemia tumours)
TSE (transmissible spongiform encephalopathies) affects mainly cattle/mink/cats.
TRUE
Prions are resistant to proteases.
TRUE
Name of TSEs (transmissible spongiform encephalopathies) in sheep
Scrapie
African Horse Sickness is caused by which virus?
Orbivirus (Sedoreovirinae)
Genera of the Picornaviridae are?
Entero / Tescho / Tremo/ Hepato / Cardio/ Aphtho / Erbo /Parecho /Kobu / Avihepato
- Alphaviruses:
a) transmitted by ticks
b) may be zoonotic
c) only present in America
d) no cross reaction.
b) May be zoonotic
Torovirus can cause?
Berne Virus (foal gastroenteritis), Breda Virus (calf gastroenteritis), Chicken , swine and human gastroenteritis
Reservoir host of Mammastrovirus?
Humans, mammals and vertebrates.
Human and bovine pathogen viruses are found in the \: a)Alpha, b)Beta, c)Gamma D) DELTA RETROVIRUS
DELTA RETROVIRUS
Prion pathogens do not contain?
Nucleic acid (proteins)
Animals are susceptible to : a)Mumps, b)Measles, c)Influenza 1? D) Nipah
Nipah
Affinity Chromatography uses?
Virus specific AB bound to chromatography column matrix. Adsorption of viruses. Rinsing. Elution buffer.
Lesions caused by Pox on CAM embryonic egg are called?
Pock lesions
- Which disease was recently eradicated from earth?
Small pox and rinderpest
How did virus get its name?
A scientist used a filter whose pores were smaller than the bacteria so the bacteria was not filtered, but following further investigation, it was found that the filtrate contained a smaller form of infectious agent. This agent multiplies only in dividing cells and it was made of particles – it was called contagium vivum fluidum (soluble living germ) and later was simply called virus.
Don’t know not arsed looking up
Prions cause what in sheep?
Scrapie
How are airborne viruses contracted?
Breathed in through the respiratory tract.
The host of Hantavirus?
Rodents
What is the given name for Goose Disease?
Goose Parvovirus (Derzsy’s Disease)
Adeno and Orthomyxo viruses - name the disease for calves.
Pneumoenteritis.
What does Aujesky Disease cause in Canines?
Lethal CNS Disease, like rabies.
Immunosuppression: True/False: Leukemia Lymphocytes Enterocytes Encephalomyelitis Don’t understand the Q – Assume correct
Leukemia TRUE Lymphocytes FALSE Enterocytes TRUE Encephalomyelitis TRUE Don’t understand the Q – Assume correct
What virus is propagated in the Chorioallantoin?
Pox and Herpesvirus
What is eclipse?
i. central step of virus multiplication
ii. from the first mRNA until the first virion assembly
iii. parts:
1. early transcription, translation: enzymes
2. replication: nucleic acid multiplication
3. late transcription, translation: structural proteins
What is it called when a virus enters the blood
Viremia
Genetic part of a virion?
DNA/RNA
The role of Rdrp in Retrovirus?
RNA Dependent RNA Polymerase) Replication of RNA.
How do bacteriophages get into the host cell?
Tailed bacteriophages = injection
T-bacteriophages = digest cell wall
What is a prion?
A prion is a small protein capable of infecting a cell and causing itself to replicate even though it contains no nucleic acid.
Virions always contain lipids
false sometimes
Virions with quasihelical nucleocapsids are enveloped
True
Virions with quasihelical capsids are never enveloped.
False
Pleomorphic capsids may not have an envelope.
False always enveloped
- Please mark which is true:
a) Mutations are more frequent in cellular organisms than in viruses
b) The effects of mutations are always advantageous for viruses
c) Mutation may alter the host specificity of a virus
d) Mutant viruses cannot be used as vaccine strains.
c) Mutation may alter the host specificity of a virus
How do we call the process when the antigenic structure of a virus suddenly changes due to reassortment?
Antigenic shift.
Polyomaviruses can cause persistent infections in kidney cells.
TRUE - goose
Papillomaviruses often cause encephalitis and diarrhoea in swine.
False
Serological cross-reactions may occur between adenovirus species within the same genus.
True
Atadenoviruses may cause disease in birds.
TRUE – egg drop disease
- Please mark the correct answer: Aujesky’s Disease is caused by the:
a) Suid herpesvirus type 1
b) Canid herpesvirus type 2
c) Porcine Parvovirus type 1
d) Porcine circovirus type 2
a) Suid herpesvirus type 1
Which virus family contains viruses with positive sense, single-stranded RNA genome and helical capsid?
Coronaviridae.
What virus causes abortions?
Varicellovirus (Aujesky’s Disease, IBR, Equine rhinopneumonitis),
Arteritis virus (PRRS, Equine Arteritis Virus),
Orbivirus (Blue Tongue, Ibraki Disease, African Horse Sickness),
Phlebovirus & Nairovirus of Bunyaviridae (Rift Valley Fever, Nairobi Sheep Fever)
How does the enveloped virus enter the cell?
Membrane fusion
Viruses can only propagate in living cells.
TRUE
What disease is caused in hens by astrovirus?
Nephritis
Which virus family causes haemorrhage?
Caliciviridae (RHD),
parvoviridae (Aleutian Mink Disease),
circoviridae (Porcine circovirus),
asfivirus (ASF),
orbivirus (African Horse Sickness, Epizootic Haemorrhagic Disease),
nairovirus (Crimean-congo haemorrhagic Fever),
Arenaviridae, paramyxovirus (Newcastle Disease),
Filovirus (Ebola).
Contact Inhibition:
When the cytoplasmic membrane edges of growing cells touches each other causing an inhibition to growth (forms a primary monolayer of cells)
Disease in hens caused by the Atadenovirus?
Egg Drop Syndrome
What do you call the infective part of the virus?
Virion. Infected cell = vegetative virus
Where do RNA viruses multiply?
In the cytoplasm (mostly)
Which virus families cause skin lesions?
Papillomaviridae, polyomaviridae, (Poxviridae cause pock lesions)
How to investigate virus neutralisation test?
Use blocking antibodies that will adsorb to the receptors of the cell so virus cant adsorb to the cell too.
- Constant virus varying serum dilution: Serial 2fold serum solution, add virus, incubate (antibodies will neutralise the virus), inoculate cell cultures, incubate, CPEs.
- Constant serum varying virus dilution: 2 Serial 10fold virus dilutions, add +and-serum, incubate, inoculate cell cultures, incubate, CPEs.
Monoclonal antibodies:
produced by a single clone of B cells •specific for a particular epitope
What proteins are found in prions?
Cellular prion protein, Infectious prion protein.
What is the genetic part of the virion?
The nucleic acid (DNA/RNA)
What is the family of the Distemper viruses?
Morbillivirus Paramyxoviridae
Haemagglutination:
This is the clumping together of red blood cells due to surface protein. Tests include: Haemagglutination test, haemagglutination inhibition test.
Concentration of a virus sample?
Precipitation, adsorption, dialysis, ultrafiltration, pelletisation.
Virus titer:
Infective titer: the highest dilution of the virus in which 50% CPEs occur.
-Haemagglutination titer: the highest dilution of the virus in which haemagglutination has not yet occurred.
Restriction nucleases:
cleave proteins at specific DNA sequences.
Cells removed from a monoculture:
Can then be used in a subculture and propagated further.
What does Aujesky’s Disease do in Canines?
Lethal CNS effects (like rabies)
What type of sample is required for Ataxia in a horse?
Conjunctival and nasal swabs, liquor cerebrospinalis, EDTA blood. (Ataxia is a neurological sign consisting of a lack of coordinating movements)
Propagation of African Swine Fever:
experimental infection of living animals
Acridin Orange Test:
Tests for the presence of either ss/ds DNA/RNA.
-Green fluorescence when bound to dsDNA.
–Red fluorescence when bound to ssDNA or RNA.
Does a greenish-yellow colour mean a doublestranded virus?
yes
Vaccines:
Live (attenuated, virulent, heterotypic, virus-vectored), inactivated, subunit, anti-idiotype. ????
Active Immunity:
Stimulation of an immune response by the body by a specific antigen (injecting a weaker live virus into the body so that the body itself must produce antibodies against the viral antigens), preventative method, long term immunity. Most vaccines do not prevent infection.
Parvo in the environment:
spread through faecal matter, but due to its very strong resistance it can survive on surfaces and be spread via contact with those surfaces.
Describe the Orthobunyavirus:
circular ssRNA with segmented genome, enveloped, - with helical capsid, viruses: Akabane disease and Schmallenburg virus of Ruminants.
Prions in sheep:
Prions are agents of Transmissible Spongioform Encephalosis and cause Scrapie in sheep.
Tick Vectors:
Colorado tick fever, Lyme disease, hepatazoonosis.
Reservoir host of Mamarenavirus?
Pet hamsters, mice (rodents)
Role of VtRt in Hepadnaviridae:
replication (Viral transcriptase Reverse transcriptase)
On which part of the virus is the lipid found
Envelope.
PCR-Colour:
Green-dsDNA, Red-ssDNA/RNA worng – blue colors look
Binary?:
induces DNA? Binary Vectors are shuttle vectors as they are able to replicate in multiple hosts.
What is the method of a hemolysis test using sheep blood?
- Haemagglutination Titre: serial 2fold dilution, add washed RBCs of appropriate species, incubate->titer is the highest dilution of virus where there is no haemagglutination as of yet.
- Haemagglutination inhibition: serial 2fold dilution of serum sample, add 4-8HA units of virus, incubate, add washed RBCs->titer is the highest dilution where there is no HA.
.Amino Acid sequence of haemagluttinating protease cleavage site of Influenza A may cause?
Will cleave the Hemaglutinin of the virus-this must be cleaved by cellular proteases to be active as a fusion protein and cause infection.
What kind of theories of origin are there?
Cell degeneraation
Runaway cell components
mRNA +ssRNA virus
chromosome fragment dsDNA virus
Which of the following is direct virus detection?
NA hybridization, isolation, IF, ELISA, PCR, Western Blot, EM,
Where do you inject Blue tongue virus?
Intravenously into embryonated hen egg
Which area of the embryonate egg do we use for haemagglutination test?
Allantoic fluid
- What does a ‘medium’ contain?
Salts, amino acids, carbohydrates, indicator, antibiotics and antifungal
What is Trypsin-EDTA used for?
Digestion between the cells of in vitro tissue culture
Are there cancer cell cell cultures?
Yes, aneuploid cell culture
What is a buffy coat?
White blood cell culture
Virus extraction methods?
. Freezing-thawing three times B. Sonication C. Detergent
With what is virus concentration by precipitation possible?
Ammonium sulphate, PEG, alcohol
What is characteristic of complex viruses?
No capsomere, complete symmetry (pox virus)
Pleomorph viruses have no nucleocapsid:
True
What can we label Nucleic acid hybridisation samples with?
Isotopes or enzymes
What can we show with PCR?
Amplification of specific DNA fragments
What is needed for sequencing?
Primer, template, deoxynucleotides, labelled dideoxynucleotides, polymerase enzyme
What cells are formed with the fusion of myeloma and B-cells?
Tetraploid hybrid cells
Non-structural proteins do not integrate into the virion
true
What is budding?
A type of enveloped virus release
Are mutations only a disadvantage?
No, they can be advantageous
Can the external environment influence mutation?
yes
Where are there more mutations?
there are 10^9 in cells and 10^3 in DNA viruses 10^3 so there are more in RNA viruses
With mutation, can species-specificity change?
yes
Are clutters thanks to/as a result of Interleukins?
No
Is autointerference autoimmune?
no
What is the reception of structural proteins
Mixing of phenotypes
Do two attenuated vaccines activate each other?
yes
Does intramolecular recombination only happen in DNA viruses
No – also RNA
Continuing from Q31) Can it happen in vacuoles and cytoplasm
yes
What causes cell cracking?
Does this mean cell rounding?
Depolarisation of the cytoskeleton
How can you send samples?
Via courier, with exact legible details, +4°C, exact address
What vectors spread rabbit haemorrhage fever?
Contact or fomites
What infects bees?
Dicistroviridae (Aparavirus: acute paralysis, acute Israeli paralysis, Kashmar paralysis, Cripavirus: black mother-pearl virus) Iflaviridae family (larvae cystic and wing deformity virus)
In horses, what causes encephalitis?
Togaviridae - alphavirus genus: Eastern, Western, Venezuelan horse encephalitis, Getah virus
Flaviviridae - flavivirus genus: Japanese encephalitis, St. Louis encephalitis, west nile
Paramyxoviridae + Paramyxovirinae - Henipavirus: Hendra virus
Bornaviridae - Borneo virus: Bornean sickness
All Bunyaviridae, are arbo viruses
False. All except hantavirus (Korean)
After taking samples, the samples have to be sent to the lab within how many hours?
Usually 24 hours (leukocytes EDTA sep: 6 hours)
When are swab samples used?
With body fluid sample
Does Border disease cause abortion in sheep?
yes
What form does herpes latency appear as in the nucleus?
Episome (ganglion, gland, macrophage, lymphocyte)
DNA microarray is a form of: NA Hyrbidization