PQ: 6.1 - 6.4 + 6.10 Forensics Flashcards
Describe the changes that occur inside a body in the fist week after death
- Body temperature falls
- rigor mortis occurs
- Digestive enzymes break down cells and tissue in the body
- Bloating
- Putrefaction
Explain how the results of this study could be used to help to establish the time of death
of a human.
Explain the effect of temperature on the rate of growth of blowfly maggots.
(3)
(i) Determine how the mean temperature of 49 °C was found.
(2)
(ii) It was observed that all the living larvae in the rhinoceros belonged to species F.
The metabolic activity of the larvae of species F increases the temperature within the
dead rhinoceros.
Explain the advantages for this species of blowfly of increasing the temperature within
the dead rhinoceros.
(3)
(i) Describe how one PCR cycle would increase the quantity of DNA present.
(3)
How do you carry out gel electrophoresis ?
- DNA from bones is cut into fragments using restriction enzymes
- Fluorescent tags are attached to the DNA
- Fragments of DNA are loaded onto the agarose gel
- Then pass an electric current through the gel.
Explain how gel electrophoresis could be used to find out if this DNA came from a black
panther.
Devise a procedure, using electrophoresis, to compare the amplified DNA from these two
types of human.
The quantity of extracted DNA is amplified using
polymerase chain reaction
Describe how DNA profiling could be carried out to show that these snakes are different
species.
(4)
Explain why the amplified DNA fragments for the G20210A allele and the wild type allele
are different.
(3)
Devise an investigation to determine the optimum number of cycles for the
polymerase chain reaction used to amplify the DNA for this test.
(4)
Comment on the effect of adding cow dung to composting coffee husks.
(3)
Explain how the results of the gel electrophoresis analysis would show whether Homo longi is closely related to other Homo species
