Post Translational Modification: Lecture 2 Flashcards
What cellular events is PTM involved in? (6)
gene expression
signal transduction
cell-cell interaction
protein-protein interaction
communication between intra and extracellular environment
What is trasncriptome?
whole mass of mrna transcripts in the body
what is PTM?
Covalent addition to or cleavage of proteins
After/ during biosynthesis
important in cell signalling
occurs on amino acid chains or at a terminal
What is proteome?
total mass of proteins in body- roughly 1 million
How are there more proteins than genes?
splicing process and PTM
what are the 3 types of germ layers that cells can differentiate into?
Endoderm- internal layer e.g. lung, thyroid, pancreatic cell
Mesoderm- middle layer e.g. cardiac, rbc, smooth muscle gut, skeletal muscle
Ectoderm- outer layer e.g. skin cells, neurone brain, pigment cells.
how does PTM relate to proteome?
PTM increase diversity of proteome whilst extending function and stability- because it affects the longevity as well as activity of protein.
Types of PTM to a protein? (5)
Glycosylation
Phosphorylation
Disulfide bond
Methylation
Acetylation
summary of glycosylation?
attach sugar- usually to N or O in amino acid side chain
summary of methylation?
add methyl group- usually at lysine or arginine residues
summary of acetylation?
add acetyl group to N-terminus of protein or at lysine residues
summary of disulfide bond?
covalent bond of S at two cysteine residues
summary of phosphorylation?
Add phosphate to serine, threonine or tyrosine
genres of PTM? (4)
cleavage- proteolysis
complex molecules- glycosylation
chemical- acetylation, methylation, phosphorylation
proteins/peptides- ubiquitylation, sumoylation
example of cleavage PTM in cell?
Insulin production. Unwanted parts of the peptide removed after translation and translocation and folding. It gets PMT with disulfide bonds (signal peptide) and then gets exported to Golgi where it’s cleaved
Whats the point of adding functional groups as PMT?
enable cell signalling and cell can react specifically and rapidly to events like environmental conditions. Fast bc can just activate/inactivate protein for rapid response e.g. insulin
key points of effects of phosphorylation? (4)
- Reversible addition/removal
- Phosphate from hydrolysis of ATP to ADP
- Cause conformational change bc phosphate -2 charge. Cause charge disparity in amino acids and change structure
- Can activate (open up/reveal active site) or deactivate (mask binding site)