Polymerases

Question 1

Type 1 restriction enzymes

Answer 1

Nuclease and methylase activity

Question 2

Type 2 restriction enzymes

Answer 2

Palindromic
Sticky or blunt ends

Question 3

Type 3 RE

Answer 3

Asymmetrical sites
Cut 24-26 bp away

Question 4

Type 4 RE

Answer 4

Asymmetrical
Cuts 10 bp away

Question 5

RNA Pol I

Answer 5

Synthesize rRNA
Translation

Question 6

RNA Pol II

Answer 6

Synthesizes mRNA
gene transcription

Question 7

RNA Pol III

Answer 7

Synthesizes tRNA and snRNA
Protein translation

Question 8

Pol I

Answer 8

Prokaryotic
Lagging strand
Proofreading
Exonuclease activity

Question 9

DNA polymerase alpha

Answer 9

Eukaryotic
Lagging strand
Primase polymerase complex
Role in Okazaki fragments
20 to 30 nucleotides synthesis

Question 10

DNA Polymerase Beta

Answer 10

Eukaryotic
Repair mechanisms
Few nucleotide additions
Hand structure
Replication restart at nicks and lesions

Question 11

DNA Polymerase Delta

Answer 11

Primary polymerase in eukaryotes
Leading strand
Proofreading
High processivity
Replication complex

Question 12

DNA Polymerase Epsilon

Answer 12

Mostly repair
Can sub for delta

Question 13

DNA Polymerase Gamma

Answer 13

Mitochondrial replication