Polymearse Chain Reaction Flashcards
1
Q
Why do we use PCR
A
PCR is used to amplify a specific series of bases
2
Q
what are the temperatures used in PCR
A
90, 50, 70
3
Q
why do we heat the DNA to 90
A
to separate the double stranded DNA and create two single strands
4
Q
Why do we cool the DNA to 50
A
to attach to the primers to the DNA
5
Q
Why do we heat DNA back up to 70
A
to extend the Primers on the strands
