Polyketide Synth

Question 1

Process of forming a fatty acid?

Answer 1

1. Acetyl CoA forms. 2. Reacts to form malonyl CoA. 3. Reacts to form CoA-S-Acetoacetate. 4. Reduced and hydrated down to longer carb.a cid. 5. Repeat

Question 2

What enzymatic domain does AT stand for

Answer 2

Acyltransferase

Question 3

What enzymatic domain does ACP stand for

Answer 3

Acyl Carrier Protein

Question 4

What enzymatic domain does KS stand for

Answer 4

Ketosynthase

Question 5

What enzymatic domain does KR stand for

Answer 5

Ketoreductase

Question 6

What enzymatic domain does DH stand for

Answer 6

Dehydratase

Question 7

What enzymatic domain does ER stand for

Answer 7

Enoyl Reductase

Question 8

What enzymatic domain does TE stand for

Answer 8

Thioesterase

Question 9

What must ACP be preceded by in the enzyme chain?

Answer 9

AT

Question 10

What must KS be preceded by in the enzyme chain?

Answer 10

ACP

Question 11

What is the role of ACP in the enzyme chain?

Answer 11

To carry the acyl unit from the upstream AT

Question 12

What is the role of KS in the enzyme chain?

Answer 12

To attach the upstream acyl unit to the downstream one.

Question 13

What is the role of KR in the enzyme chain?

Answer 13

To reduce ketones to secondary alcohols on downstream ACP

Question 14

What is the role of DH in the enzyme chain?

Answer 14

To reduce alcohols to alkenes on downstream ACP

Question 15

What is the role of ER in the enzyme chain?

Answer 15

To reduce double bond to single bond on downstream ACP

Question 16

What is the role of TE in the enzyme chain?

Answer 16

To release and/or cyclise the final polyketide