POCT Flashcards
alternative for when alcohol cannot be used.
Benzalkonium chloride
(determining lead, mercury)
- Trace element free tube (royal blue top)
why is - Trace element free tube (royal blue top) necessary
kase regular tubes have traces of heavy metals
- ARD
Anticoagulant ruling device
- ARD
Anticoagulant ruling device
ARD can be made of
o Can be made of plastic resins (already incorporated blood culture bottles) / activated charcoal (already incorporated in
o for blood culture whenever patient is already taking antibiotics
- ARD (Anticoagulant ruling device)
o Used to neutralize the effect of antibiotic. Removes antibiotic residue
ard
= presense of bacteria in blood. Normally, blood should be sterile
bacteremia
when bacteria is already multiplying in the blood stream
o Septisemia
o For drug testing.legal issue kase to. Discerns lahat na humawak ng sample from the collection of urine till release of result. Usually urine sample. Iallagay observations kung nagkakaran leakage kunwari
ccf
ccf
chain of custody form
body has excess of blood. Increase of rbc. Malapot madalas.
polycthemia vera
body has excess of blood. Increase of rbc. Malapot madalas.
polycythemia vera
ANTICOAGULANTin blood bag
ACD or CBD or CBDA.
LACTATE IS NOT DONE FOR BLOOD CULTURE
o Lactate test = usually done in blood culture.
use of ice in lactate test
o Ice is for preserving lactate during transfer in lab
why is ice tube in lactate test wrapped in double bag
o Iced tube wrapped in double bag para kahit na dumikit sa container di mahehemolyze dugo.
o In other method need 14mm mercury blood pressure to do the bleeding and clotting time
- Sphymomanometer
- Performed to diagnose septecimia or bacteremia
blood culture
specimens for blood culture collection is collected in
- Specimens collected in special bottles containing culture medium (nutrient broth) aerobic and anaerobic bottles
bacteria that are able to tolerate oxygen
aerobes
bacteria that dies in presence of oxygen which can often be cause of bacteremia and septesemia ()
anaerobes
anticoagulant in blood culture
SPS (sodium polyanethol sulfonate)
- Blood specimens for patients unedrsgoing antimicrobial therapy are collected
using ARD for patients under antimicrobial therapy
- One of the most common error in blood culture is
contamination
2 disinfectant in blood culture
alcohol and povidone iodine
contact time for iodine in disinfecting blood culture
1 min
- In blood culture pag needle and syringe
anaerobic muna. Long term exposure adds exposure to oxygen.
Butterfly method
- = aerobic muna. Kase tubing may air that could contribute to level of oxygen.
INCUBATION TIME BRUCELLOSIS
4wks
INCUBATION TIME tb
6wks
INCUBATION TIME
Anaerobic infections
14days
Normal time for observation
INCUBATION TIME
5DAYS
Normal time for observation
LACTATE
gets higher during episodes of low oxygen level (in strenuous exercise, severe infection like sepsis kaya ginagawa alongside blood culture)
LACTATE OR LACTIC ACID
DISORDER
Bleeding or Thrombin time
thrombocytopenia, von willebrand disease
disorder
Platelet count
thrombocytopenia
disorder
PT
(prothrombin time)
defect in vitamin k-dependent factor, liver disease, DIC
APTT
(activated partial thromboplastin time)
disorder
haemophilia, von willebrand disease, DIC
MECHANISM TESTED
Bleeding or Thrombin time
Hemostasis, capillary and platelet function
MECHANISM TESTED
PLATELET COUNT
Platelet number
MECHANISM
PT
Extrinsic and common pathway
mechanism tested
aptt
Intrinsic and common pathway
pt and aptt ratio
9:1
- In ? method need sphygmomanometer
hibis
bleeding time retdem method
deux
in bleeding and clotting time, puncture should have
- Puncture should not exceed 2.4 in width . depth not more than 3mn,
bleeding time normal value
2-4/6
clotting time normal value
2-8 mins
konti cells napoproduce sa bone marrow)
bone marrow aplasia
platelets not bind ng maayos)
Defective platelet aggregation
PROLONGED BLEEDING TIME IN:
Thrombocytopenia (kulang platelet)
Bone marrow aplasia or bone willebrand disease (konti cells napoproduce sa bone marrow)
Defective platelet aggregation (platelets not bind ng maayos)
PROLONGED CLOTTING TIME IN:
Liver diseases (production of clotting factors) _ (coagulation factor are synthesized by liver)
Anticoagulant therapy (takes anticoagulant)
Vitamin K deficiency (defected clotting factors 2,7,9 and 10)
Detection of gestational diabetes
OGCT
- 75g glucose solution
OGCT
- Confirm screening positive for fbs or rbs
- Determines how quickly glucose is cleared from the blood
OGTT
- 100g solution
OGTT
- 8-12 hours fasting. Avoid exercise and
- hemolyzed sample not allowed
OGTT
MEANING OF OGTT TEST RESULT
<140
NORMAL
140 to 200
(7.8-11.1mmol/l)
MEANING OF OGTT TEST RESULT
Pre-diabetes
200>
MEANING OF OGTT TEST RESULT
diabetes
OGCT TEST RESULLT ILAGAY D2
N
exposed to sugar found in circulation for as long as rbc life span (90-120 days). Didikit ditto yung sugar
hemoglobin a1
- Kung ano level ng sugar sa circulation for 30 or 90 days that will be the average glucose level of the patient during the 90 days.
hemoglobin a1
- Like fbs but sample is plasma not serum
fpg fasting plasma glucose
- a unit of blood from a patient is removed and not replaced. It is used as a treatment for polycythemia vera
blood donor collection
autologous blood donation
for specific person. for operation?
- testing of drug levels at specific intervals to help establish a drug dosage to avoid drug toxicity
THERAPEUTIC DRUG MONITORING
- monitoring of drug should be at
through or peak
- = drug level collected when the lowest serum concentration of the drug is expected, usually prior to the administration of the next scheduled dose
TROUGH LEVEL
drug level collection at its highest serum concentration of the drug during a dosing interval
peak level
- = a paper trail of docx to track and document each step in the drug testing process and each responsible entity that touches the specimen from the time it was submitted by the donor up until the results are released
chain fo csutody form
ml of urine in drug test
60ml
confirmatory methods of drug test
gcms
- = “bedside testing” is defined as medical diagnostic testing at or near the point of care-that is, at the time and place of patient care
POCT