Platelet Gel

Question 1

Dense granules contain…

Answer 1

ADP
ATP
Serotonin
Calcium

Question 2

Young/dense vs. old/light plateletsq

Answer 2

Young aggregate 3-5x faster
Young release 4-8x more ATP and 4-6x more ADP
Old platelets require more ADP to be activated

Question 3

Primary hemostasis

Answer 3

Vasoconstriction
Platelet adhesion
Platelet aggregation

Question 4

Secondary hemostasis

Answer 4

Activation of coagulation factors

Fibrin formation

Question 5

Platelet derived growth factor

Answer 5

Strongly mitogenic and chemotactic for leukocytes

Doubles rate f collagen deposition in wound

Question 6

Transforming growth factor beta

Answer 6

Strongly mitogenic

Allows damaged tissue to return to normalized collagen deposition

Question 7

CD34 protein

Answer 7

Concentrated in mononucleated layer of platelets

Stem cell markers important for adhesion and chemotaxis

Question 8

Platelet gel contraindications

Answer 8

Severe hypovolemic
Unstable angina/LM disease 
Heparin therapy 
Post incisional harvest
Thrombocytopenia (

Question 9

Places to find mesenchymal stem cells

Answer 9

Bone marrow most common
Circulating blood
Cord blood
Fallopian tubes
Fetal tissue

Question 10

Actions of Adipose derived stem cell extraction devices

Answer 10

1. Remove lipids
2. Remove supernatant (saline, epinephrine, lidocaine)
3. Concentrate adipose stem cells
4. Maintain stromatolites vascular fraction to create warm, fuzzy micro environment to promote graft retention
5. Allow for stem cell extract to be mixed at same ratio with platelet concentrate in anaerobic environment

Question 11

Alpha factors contain…

Answer 11

Clotting factors
Growth factors
Other proteins