Plasmid

Question 1

What is RNA 1?

Answer 1

• A negative regulator

Question 2

What is RNA 1’s function in ColE1?

Answer 2

• Binds with RNA 2 to stop further replication occurring.

Question 3

How is RNA 1 regulated?

Answer 3

• Its concentration is proportional to the copy number of the cell (More plasmid = more RNA 1)

Question 4

What is the complex called when RNA 1 and RNA 2 bind?

Answer 4

• Kissing complex

Question 5

What binds RNA 1 and RNA 2?

Answer 5

• Rom and Rop protein

Question 6

What occurs after RNA 2 binds to origin of rep?

Answer 6

• RNA 2 DNA hybrid allows RNA 2 processing, RNase H binds, polymerase binds, Primer maturation, Replication

Question 7

What is plasmid incompatibility?

Answer 7

• Two plasmids with the same origin of replication have the same system for copy number. The regulatory system cant tell them apart, therefore one is lost to chance.

Question 8

How do you avoid plasmid incompatibility?

Answer 8

• Make sure the plasmids have different origin of rep and Ab resistance markers so can differentiate between them.