Plasma Proteins

Question 1

Time for clot retraction

Answer 1

1-2 hours

Question 2

3ml of blood gives ____ ml serum

Answer 2

1

Question 3

What is serum

Answer 3

Defibrinated plasma
Lacks coagulation factors Inc prothrombin and fibrinogen

Question 4

Total protein content of normal plasma

Answer 4

6-8g/100ml

Question 5

A
G
F
Levels

Answer 5

3.5 -5 g/dl
2.5-3.5g/dl
200-400 mg/dl

Question 6

A:G

Answer 6

1.2:1 - 1.5:1

Question 7

Synthesis of plasma proteins

Answer 7

In liver
Except immunoglobulins

Question 8

Fibrinogen is pptd by

Answer 8

10% sodium sulfate

Question 9

Globulins pptd

Answer 9

22% sodium sulfate

Question 10

Ammonium sulfate will ppt

Answer 10

Globulin - half saturation (solution)
Albumin - full saturation (crystals)

Question 11

In clinical laboratory total proteins in serum or plasma of patients are estimated by

Answer 11

Biuret method

Question 12

Albumin Is qhantitated by

Answer 12

Bromocresolgreen method in which dye is preferentially bound w albumin and the color is estimated colorimetrically

Question 13

Separation of plasma proteins in clinical laboratory

Answer 13

SPE

Question 14

SPE (6)

Answer 14

Electrophoresis - movement of charged particles through an electrolyte when subjected to an E.

Anions migrate from negative (cathode) to positive pole (anode)

Rate of migration depends on charge and mol.wt

5 fractions usually separated by SPE

Albumin having max negative charge and least molecular weight migrates maximum

5 fractions are qhantitated by scanning and measurement of their intensity