plant stems

Question 1

method

Answer 1

1] Place a small piece of tinned rhubarb on a watch glass. 2] Use forceps to pick out one or two vascular bundles from this block of tissue and place them on a microscope slide.
3]Use mounted needles to tease the vascular bundles apart. Cover the tissue with a drop of methylene blue, and leave for 5 minutes.
4] Draw off the extra stain with filter paper. Place a drop of dilute glycerol on the fibres and mount under a coverslip.
5] Examine your preparation under low, medium and high magnification.
To re-irrigate the slide with glycerol, place a drop of glycerol on the slide next to the coverslip. It will be drawn under the coverslip by capillary action. Blot off any excess and re-examine the slide.
6]Look for vascular bundles amongst the separated tissues.

Question 2

observations

Answer 2

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