PINAUD EXAM 2 Flashcards

1
Q

basic characteristics of enzymes

A

increase rate by lowering AE
do not alter equilibrium constant
often require co-factors
usually proteins, sometimes RNA
highly specific to substrate and reaction

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2
Q

protease

A

catalyzes the hydrolysis of protein peptide bonds

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3
Q

thrombin

A

proteolytic enzyme in blood clotting
Cuts between Arg and Gly

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4
Q

trypsin

A

enzyme in the digestive system
cuts after Arg or Lys

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5
Q

holoenzyme

A

apoenzyme (inactive) + cofactor (coenzyme or metal)

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6
Q

∆G equation

A

∆G = ∆G° + RT ln [products]/[reactants]

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7
Q

Keq equation

A

kf/kr

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8
Q

3 ways to increase the rxn rate (k)

A

increase substrate conc.
increase T
decrease activation energy

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9
Q

ES complex characteristics

A

shape of active catalytic pocket is 3D (via steric hindrances of AA residues) and flexible, often nonpolar
induced fit: change conformation after binding
multiple weak interactions between E and S (H bonding, electrostatic, hydrophobic, VDWs)

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10
Q

transition state

A

short lived chemical state
highest peak of ∆G diagram
strong binding and flexibility of ES complex promotes formation of transition state

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11
Q

kinetic evidence for ES complex

A

rxn rate increases with increases substrate conc until a plateau (enzyme conc)

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12
Q

physical evidence for ES complex

A

x-ray crystallography

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13
Q

binding energy

A

some free energy released upon binding ES, helps form active site and lowers ∆G of transition state

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14
Q

enzymes speed up biochemical rxns by…

A

specific substrate recognition
multiple reactive steps at catalytic site
strong binding to transition state
efficient release of product

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15
Q

first order rxn

A

V = k[S], units s-1

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16
Q

second order rxn

A

V = k[S][B], units M-1 s-1

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17
Q

at low [S]…

A

Vo proportional to [S]

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18
Q

at high [S]…

A

Vo independent of [S]

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19
Q

Km

A

substrate concentration at 1/2(Vmax)

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20
Q

kcat

A

turnover rate (molecules/s), only works when Vmax has been reached

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21
Q

Michaelis-Menten equation

A

Vo = Vmax ([S]/[S] + Km)

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22
Q

what does Km say about the strength of ES complex?

A

low Km = stronger binding
high Km = weaker binding

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23
Q

enzyme efficiency measurement

A

kcat/Km
10^8 to 10^9 is catalytically perfect

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23
Q

lineweaver burke plot

A

reciprocal of Michaelis-Menten curve, linear
1/Vo = (Km/Vmax)(1/[S]) + 1/Vmax

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24
types of reversible enzymatic inhibition
competitive, uncompetitive, noncompetitive
25
irreversible enzymatic inhibition
tight binding to enzyme
26
competitive inhibition
enzyme binds to S OR I enzyme freed from I by increasing [S] increases Km, Vmax unchanged
27
DHFR (dihydrofolate reductase)
needed for cell division methotrexate (similar structure) = competitive inhibitor to DHFR, 1000x tighter binding, cancer drug
28
methotrexate
competitive inhibitor to DHFR (structurally similar) 1000x tighter binding than DHFR cancer drug used to kill rapidly dividing cells
29
uncompetitive inhibition
I binds after S ESI complex cannot make P Vmax decreases and cannot be attained Km decreases High [S] does not overcome inhibition
30
noncompetitive inhibition
I and S bind at the same time ESI cannot make P Vmax decreases high [S] does not overcome inhibition
31
4 types of irreversible inhibition
group specific modifying agent, affinity labels, suicide inhibitors, transition state analogs
32
group specific modifying agent
react with specific group at modifying site
33
affinity labels
inactivate enzyme by covalent modification
34
suicide inhibitors
chemical mechanism makes enzyme react covalently with inhibitor
35
transition state analog
similar to transition state structure, binds more strongly to E than S
36
proline racemase
enzyme that catalyzes isomerization of proline pyrrole 2-carboxylic acid acts as transition state analog to planar proline ion (transition state)
37
penicillin
transition state analog and suicide inhibitor inhibits glycopeptide transpeptidase (forms bacterial cell walls through peptide cross-linking)
38
statins
competitive inhibitors of HMG-CoA reductase (cholesterol synthesis) similar structure to substrate inhibits cholesterol synthesis
39
reactive cleft
environment favoring S + E interaction close proximity of substrate to active reading groups optimized orientation of substrate for rxn rxn protected from water / hydrolysis
40
aspirin
irreversible covalent inhibitor of prostaglandin H2 synthase (prostaglandin synthesis --> transmission of pain info, inflammation) acetylation of serine residue in channel to reach active site - anti-inflammatory
41
induced fit
stabilizes various conformations for both E and S optimized orientation of catalytic groups in enzyme allows very tight binding to transition state (gives free energy to accelerate catalysis)
42
covalent catalysis
reactive groups of enzyme become covalently attached to substrate covalent E-S bond highly reactive for next step usually involves strong Nu-
43
acid-base catalysis
reactive groups of enzymes donate or accept a proton involves acidic and basic AA residues
44
metal ion catalysis
loosely (Ca2+) or tightly (Zn2+) bound to enzyme ionic interactions with substrate or enzyme groups shield neg charges or stabilize charges
45
serine protease
uses Ser 195 as a highly reactive group for catalysis transient covalent interaction acetylation & deacetylation
46
oxyanion hole
area of active catalytic site that tightly binds tetrahedral transition intermediate, stabilizes O- charge
46
catalytic triad
making Ser 195 a Nu- H-bound network between Asp 102, His 57, Ser 195 mutations within triad lead to dramatic decrease in catalytic efficiency
47
chymotrypsin
cuts after bulky hydrophobic AAs, Trp, Phe, Met pocket is deep and hydrophobic
48
trypsin
cuts after long positive AAs Lys and Arg Asp (-) at the bottom of pocket
49
elastase
cuts after AAs with small side chains, Ala and Ser narrow pocket
50
cysteine protease
catalytic mech resembles Ser triad
51
Aspartyl protease
use Asp carboxylate group to activate H2O and attack peptide bonds
52
Metalloproteases
use metal ion to activate H2O ex. carbonic anhydrase
53
proton shuttle
His 64 removes proton in carbonic anhydrase catalysis to achieve fast catalytic rates
54
"committed step"
first step that makes reaction irreversible, feedback regulation will target the product of the first committed step
55
homotropic effects
caused by substrate itself at catalytic sites increase catalytic rates
56
heterotropic effects
caused by binding of non-substrate ligands decrease catalytic rates
57
R + T states
relaxed and tense states of an enzyme, rapid switching
58
cooperative binding
binding of 1 substrate causes increased binding affinity for another
59
ATCase
allosteric enzyme involved in synthesis of pyrimidine nucleotides
60
end product for ATCase?
CTP, which causes negative feedback on ATCase
61
structure of ATCase
12 subunits: 6 catalytic and 6 regulatory
62
PALA
substrate analog, binding to ATCase causes large conformational changes equilibrium toward R state (basically a model to understand substrate binding and how it affects catalytic activity)
63
CTP
allosteric inhibitor of ATCase binds to regulatory subunits in T state, stabilizes low catalytic efficiency, equilibrium toward T, decrease ATCase affinity for substrates
64
ATP (in ATCase)
allosteric effector of ATCase competes with CTP on regulatory subunits favors R state and pyrimidine synthesis
65
isozyme
multiple forms of an enzyme catalyzing the same reaction in different ways or in different tissues long-term regulation (M and H isozymes of LDH based on aerobic conditions throughout lifespan in rats)
66
kinase and phosphatase
kinase > phosphorylates (transfers phosphate group from ATP to AA residue), signal amplification phosphatase > dephosphorylates (hydrolysis of phosphate ester) cascade amplification effects!
67
Protein Kinase A (PKA)
phosphorylates many proteins! 2 C 2 R subunits activated by cAMP
68
PKA consensus sequence
Arg - Arg - small AA - Ser/Thr - large AA
69
PKA substrates
1) consensus seq / pseudosubstrate (inactive) 2) ATP (phosphorylation cascade)
70
PKA shabangle
cAMP binding > conf change > frees C subunits (pseudosubstrate) > activates PKA > binds ATP > phosphorylation cascade
71
amplification example
1 cAMP causes a lot of phosphorylation adrenaline causes a lot of G-protein to bind
72
zymogens
proenzymes/inactive forms of enzymes activated by proteolytic cleavage ex. proteolysis of trypsin activates chymotrypsin
73
reduction in sugars
hemiacetal - open - OH - reducing acetal - locked - OC - nonreducing
74
non-reducing sugar
sucrose
75
reducing sugars
lactose and maltose
76
lactose
galactose + glucose
77
maltose
glucose + glucose
78
glycogen
glucose polymer a-1,4 linkages (favor packaging), some a-1,6 for branching reduce osmotic pressure maintain glucose inside cell
79
cellulose
glucose polymer B-1,4 linkages (fiber like structure for strength) humans can't digest these linkages no branching
80
glycoproteins
protein modified by carbohydrate at specific AA residues via glycosyltransferase ER and golgi
81
glycoprotein linkages
N-glycosidic linkage with Asn in Asn-X-Ser/Thr with X=any but proline O-glycosidic linkage with Ser or Thr
82
erythropoietin (EPO)
blood glycoprotein involved in stimulating RBC production (higher number RBC, limit degradation)
83
recombinant EPO
helpful in treating anemia but also in blood doping can be detected in blood due to different glycosylation pattern
83
blood types
based on glycosylation patterns glycosyltransferase enzymes expressed by body define type
84
mucins
highly glycosylated proteins, act as lubricants in secretions (saliva, mucus) Ser and Thr o-linked glycosylations localized in VNTR domain
85
O-antigens
common oligosaccharide foundation
86
A-type glycosyltransferase
adds N-acetylgalactosamine
87
B-type glycosyltransferase
adds galactose
88
fatty acids
14-24 hydrocarbon chain w carboxyl
89
saturation of fatty acids
saturated = higher MP, less fluid unsaturated = lower MP, more fluid
90
classes of membrane lipids
phospholipids (glycerophospholipids, sphingolipids) glycolipids (sphingolipids) cholesterol
91
glycerophospholipid
glycerol connected to 2 FA chains and PO4 - alcohol
92
phosphosphingolipid
sphingosine connected to 1 FA and PO4-choline
93
glycosphingolipid
sphingosine connected to 1 FA and mono/oligosaccharide
94
cholesterol structure
4 rings, 1 hydroxyl, 1 alkyl chain parallel to other lipids with OH interacting with heads
95
arrangement of fatty acid chains
micelle: single fatty acid chain liposome: closed lipid bilayer (circle) formation driven by hydrophobic int, close packing by VDWs
96
liposomes
can be used as nanocontainers and reactors thermodox: targets cancer tissue, heat releases toxins
97
permeability of lipid bilayers
low permeability to ions and polar molecules (except water) highly selectively permeability barriers to ions using protein pumps/channels, maintains ion conc gradient
98
membrane protein functions
transport across membranes signaling/transfer info maintain electric potential
99
lipid : protein ratio in membranes
varies from 1:4 to 4:1
100
3 major types of membrane anchoring
transmembrane (very strong association) electrostatic interaction (weak) lipid anchoring (strong)
101
alpha helices
7-span proteins 20 AA/helix extensive H-bonding formed of hydrophobic AA residues
102
bacteriorhodopsin
light driven proton pump used by halobacteria, alpha helix
103
hydropathic index
uses ∆G when transferred from hydrophobic to aqueous environment each point measures the AA window average ∆G (slides 1) ∆G > 0 hydrophobic ∆G < 0 hydrophilic only detects alpha helices not B sheets! alpha helix criterion level of +84
104
beta sheets
common for pore forming proteins channel protein extensive H-bonding, barrel structure alternates between hydrophobic and philic AA external: hydrophobic, internal: hydrophilic
105
prostaglandin H2 synthase
peripheral protein that converts arachidonic acid into prostaglandin in ER membrane a-helix hydrophilic backbone for partial attachment, hydrophobic channel for arachidonic acid to get to active site
106
FRAP (fluorescence recovery after photobleaching)
shows that membrane is dynamic after bleach, rapid lateral diffusion in the plane of the membrane for both lipids and proteins recovery time as a measurement of membrane fluidity
107
what affects the overall melting temp of membranes?
length of fatty acids and degree of unsaturation saturation takes priority over length
108
Tm
melting T of membrane (solid to fluid)
109
how does cholesterol influence membrane fluidity?
increases fluidity in low temperatures by disrupting tight packing decreases fluidity in high temperatures by interfering with kinetic fatty acids
109
asymmetry in membranes
outer and inner membranes differ in lipid composition and associated proteins maintained because flip-flopping is rare and requires flippase orientation of transmembrane proteins do not change over time outer membrane rich in glycosylated proteins and lipids
110
uncharged membrane permeable molecules
chemical concentration gradient determines spontaneous movement across membrane
110
charged membrane permeable molecules
chemical concentration gradient AND electrical potential term determines movement across molecule
111
∆G trans
determines if the transport across a membrane is passive or active
112
membrane pumps
primary active transport drive thermodynamic uphill reactions with ATP ATP causes conformational changes for open and close
113
membrane carriers
secondary active transport drive thermodynamic uphill reactions using chemical gradient of one molecule to drive transport of a second molecule against own gradient no ATP
114
membrane channels
passive transport selective pore can be sensitive to membrane polarization
115
What is SERCA ATPase: Ca2+ pumps
rapidly removes excess Ca2+ after Ca2+ triggered muscle contraction needs ATP P-type ATPase 10 transmembrane a-helices and 2 domains (A, P, N)
116
SERCA pumps mechanism
2 Ca2+ / ATP 1) 2 Ca2+ bind transmembrane domain from cytoplasm 2) ATP binds N domain 3) P domain phosphorylated on Asp residue 4) CC by A domain --> Ca2+ release into SER lumen 5) phosphoaspartate residue hydrolyzed (dephosphorylation) 6) transmembrane domain conformation reset
117
ABC (ATP binding cassette) transporter pumps
membrane pumps: 1 transmembrane domain and 2 ABC domains Exist in equilibrium between open and closed conformations Substrate binding stabilizes weak closed state, enhances affinity for ATP binding ATP binding induces strong interaction between ABC domains > CC > substrate release Reset of ATP pump by hydrolysis
118
types of cotransport
antiporter: 2 solutes in opposite directions symporter: 2 solutes in same direction no ATP
119
lactose permease symport
lactose permease in E. Coli uses H+ gradient to drive entry of lactose against H+ binding favors lactose binding > CC > lactose release > proton release in cell
120
fastest transporters
1000 folds faster than pumps or carriers, close to diffusion rates of ions
121
action potential
generated in neurons by membrane depolarization involving Na+ and K+ ion channels
122
patch clamping
study ion channels by isolating single channel with a pipette, record changes in current upon opening and closing
123
ion specificity in K channel
1) sequence specific peptidic backbone TVGYG provides polar interactions with K+ as replacement for H2O, TVGYG optimal for K+ only and tight binding 2) electrostatic repulsions between K+ ion in channel allows for rapid flow
123
structure of K channel
tetramer forming pore through lipid membranes larger solvated entry into channel, then narrow channel constriction forces passing K+ ion to desolvate (loos H2O) (funnel-like)
124
voltage gating
channel will open given a specific change in membrane potential
125
ligand gating
channel will open upon binding of specific ligand
126
voltage gating in K+ channels
S4++ domain at the bottom of the channel is positively charged close channel: S4++ domain in the down position open channel: membrane depolarization causes S4++ to go upward due to electrostatic repulsion, channel opens
127
ball and chain model
small peptide fragment is attached by a flexible domain, in open state it occludes the pore and inactivates transport very quick, time depends on chain length
127
acetylcholine receptors
responsible for electrochemical signal transduction at synapses binding of acetylcholine triggers opening of non-selective channel, Na+ and K+ travel freely, membrane depolarization (-60 to -20)
128
gap junctions
large channels cell to cell communication long opening times controlled by membrane potential and phosphorylation
129
action potential mechanism
1) neuron firing > acetylcholine release in synaptic cleft 2) acetylcholine binding to receptors open ligand-gated channels 3) entry of Na+ and exit of K+ ions 4) at -40 mV, Na+ channels (voltage gated) open, more Na+ enters, further depolarization 5) Na+ channels inactivate, K+ channels open 6) K+ efflux repolarizes membrane, K+ channels inactivate 7) Na/K pumps use ATP to return to resting potential
130
aquaporins
rapid H2O transport 6 a-helices form hydrophilic channel
131
B-Adrenergic Receptor (B-AR) signal transduction
1) Adrenaline binds β-AR, activating receptor 2. Heterotrimeric G-protein (G⍺sβƔ) binds receptor and exchanges GDP for GTP 3. G-protein (β + Ɣ) subunits disassociate and G⍺s subunit binds downstream effector adenylyl cyclase (AMP 1: 1 B-AR to many G⍺s) 4. Adenylyl cyclase converts ATP to cAMP (AMP 2) 5. cAMP activates PKA triggering a phosphorylation cascade and activation of other effectors (AMP 3)
132
Reset of G protein
hydrolysis of GTP to GDP reassociation of G-protein units
132
reset of B-AR receptor
dissociation of ligand (adrenaline), phosphorylation of C-terminal tail allows binding of B-arrestin, desensitizes the receptor
132
angiotensin II receptor signal transduction
1) G⍺q-GTP binds, activates PLC 2) PLC binds PIP2, cleaves into IP3 and DAG 3) IP3 releases Ca2+ from ER, DAG activates PKC after it receives Ca2+
132
insulin receptor signal transduction
1) insulin binding > cross-phosphorylation by tyrosine binding on an activation loop 2) IRS bind receptor, bind PIP2 lipids with a pleckstrin domain, IRS phosphorylated 3) PIP3 Kinase recruited, makes PIP2 > PIP3 4) PIP3 activates PDK1 5) PDK1 phosphorylates Akt > cascade 6) storage of glucose via glycogen synthesis
133
calmodulin
Ca2+ binding domains called EF hands, large CC after Ca2+ binding
133
EGF receptor signal transduction
EGF stimulates cell growth 1) EGF binding > dimerization of receptor > CC 2) tyrosine kinase activity for cross-phosphorylation 3) recruitment of Grb-2, then SOS 4) Ras activated by GTP binding Ras binds and activates kinases for P cascade
133
Ca2+ as an intracellular messenger
stored in ER, low cytoplasmic Ca2+, binding of Ca2+ to calmodulin = CC and signal transduction
133
adapter proteins
specialized domains (SH2, pleckstrin domain, SH3), needed for precise localization of signal transduction
134
kinase examples
PKA, PKC, PIP3K, PKB receptors themselves can have kinase activity: insulin and EGF
134
second messengers
provide delocalization of signals in cell, fast amplification effects Ca2+, cAMP, IP3
134
negative feedback mechanisms in signal transduction pathways
clocked deactivation (GTPase activity in G-protein, Ras) general deactivation by phosphatase ligand release of receptor internalization
135
Cancer and signal transdutions
gene encoding important proteins for signal transduction mutated Ras gene often mutated, trapped in active form with lost ability to hydrolyze GTP > uncontrolled cell growth
136
cholera
toxin alters G-protein activity Gas constantly active, continuous PKA activation opening of Cl- channels excessive loss of H2O and Na+, diarrhea/dehydration/death
137
myosin structure
two heads (motors), one long shaft P-loop ATPase cores, 2 light chains
138
myosin substrate
ATP-Mg2+
139
myosin V ATPase activity
ATP hydrolysis by nucleophilic attack of H2O (takes off 1 phosphate) vanadium replaces phosphoryl group dephosphorylated = swinging lever-arm motion, power stroke state
140
actin binding of myosin, L T Head specific!
S1 heads (L and T) bind actin filaments CC change favors release of Pi 1) T empty (strong actin binding) while L binds ADP + Pi (weak) 2) T binds ATP (detached) 3) ATP does to L head as ADP + Pi ATP attachment and detachment only from T head
141
two models for myosin V movement
hand over hand: like a little human inchworm: scoochies
142
structure breakdown of muscle fiber
thin actin filaments and thick myosin > sarcomeres > myofibrils > muscle fiber each myosin filament binds 2 actin filaments on either end so able to contract
143
muscle contraction mechanism
1) ATP binds to myosin head, myosin releases actin 2) ATP > ADP + Pi, myosin head cocks to high energy conformation 3) Pi released from myosin, CC > myosin power stroke 4) ADP released
144
regulation of actin binding
1) action potential causes Ca2+ release 2) Ca2+ binds to troponin on actin, displacing tropomyosin 3) myosin heads can latch to actin
145