photosynthesis chromatography

Question 1

A student obtained a solution of pigments from the leaves of a plant. Then the student used paper chromatography to separate the pigments.

The diagram shows the chromatogram produced.

(c)  Explain why the student marked the origin using a pencil rather than using ink.

Answer 1

Ink and (leaf) pigments would mix

OR

(With ink) origin/line in different position

OR

(With pencil) origin/line in same position

OR

(With pencil) origin/line still visible

Question 2

Describe the method the student used to separate the pigments after the solution of pigments had been applied to the origin.

Answer 2

  Level of solvent below origin/line;

Reject water or any named aqueous solution.

Accept named organic solvent.

2.   Remove/stop before (solvent) reaches top/end;