Photosynthesis Flashcards
Photosynthesis
Conceptually, photosynthesis is the reverse of cellular respiration.
- Solar energy is converted into chemical energy in the form of chemical bonds in organic molecules
- Photosynthesis occurs in the plant cell chloroplasts
Chloroplasts
Thylakoid membrane contains chlorophyll that absorbs light energy for photosynthesis
Photosynthesis reaction
In the presence of sunlight, green plants produce oxygen and food from carbon dioxide and water.
Photosynthesis vs. Respiration
- Photosynthesis occurs in the chloroplasts (endergonic)
- Occurs in the mitochondria (exergonic)
Movement of Oxygen
- CO2 oxygen goes to glucose oxygen (carbon fixation). Solar energy is used to reduce CO2 into sugar by transferring electrons together with H+ from H2O to CO2 (thuse photosynthesis is a redox reaction)
- H2O oxygen goes to O2 (splitting of water). Therefore, the oxygen comes from the splitting of H2O, and H+ is then used to produce food
Light Reactions
Convert solar energy into NADPH and ATP and release molecular oxygen (splitting of water)
- Require light
- ATP is made by photophosphorylation
- NADPH made by transferring an electron (and H+) from H2O to NADP+. Also releases molecular oxygen as by-product.
- No sugar is produced during light reactions
- ATP and NADPH are then used in the Calvin cycle to make sugar
Calvin Cycle (general)
Converts CO2, ATP and NADPH into sugar (carbon fixation)
- Does not require light
Why are chloroplasts green?
Plants absorb Blue and Red light and transmit Green light (therefore they are green).
- Chloroplasts have 3 major types of pigments: chlorophyll a, chlorophyll b, and carotenoids
Structure of chlorophyll
- Porphyrin ring with MG atom (light absorbing region)
- Hydrophobic tail anchors it to the thylakoid membrane
Photoexcitation of chlorophyll
Chlorophyll a
- Ground state = electron in the normal orbital
- With light => excited state = electron goes to a higher orbital
- Electron is transferred to the primary acceptor => ATP and NADPH synthesis
- In an isolated system, the “fall” of the electron from the excited to ground state releases energy (heat + fluorescence)
- In nature, the energy is not lost; electrons are transferred to other molecules
Photosystem
Step 1. Light Step 2. Pigments - Antenna: -> Many pigment molecules (chl a, chl b, carotenoids) -> Farther from the primary acceptor -> Gather light and transfer electrons to reaction center - Reaction Center: -> 2 molecules of chlorophyll a -> Close to the primary acceptor -> Transfers e- to the primary acceptor Step 3: Primary electron acceptor - Receives e- from reaction center and transfers them to reactions of ATP/NADPH synthesis
Two Photosystems
- Photosystem 1 (P700)
- Reaction center has chlorophyll a that absorbs light at 700nm - Photosystem 2 (P680)
- Reaction center has chlorophyll a that absorbs light at 680 nm
- Differences between P700 and P680 chlorophylls a: they are associated with different proteins
How do photosystems work?
They transfer electrons by:
- Cyclic electron flow:
- Only in photosystem 1
- Only ATP is made - Non-cyclic electron flow:
- Both in photosystem 1 and 2
- ATP and NADPH are made
Cyclic electron flow
- No NADPH or O2 is synthesized
- H+ gradient is made by chemiosmosis and it drives the ATP synthase to make ATP
Major Steps in non-cyclic electron flow
Step 1:
- Photosystem 2 absorbs light (photons)
- Electrons in chlorophyll are excited
- Excited electrons are transferred to the primary acceptor of photosystem 2
- Because chlorophyll of photosystem 2 lost an electron, it needs to be reduced back
Step 2:
- An enzyme splits water into H+ and 1/2 O2
- Electrons are then transferred back to chlorophyll of photosystem 2, reducing it
- 1/2 O2 combines with another oxygen atom to make O2 which is then released
Step 3:
- Electrons pass from the primary acceptor of photosystem 2 through ETC and release energy used to make ATP by photophosphorylation (this ATP is used to drive the biosynthetic Calvin cycle)
Step 4:
- After ETC, electrons originating from photosystem 2 go to photosystem 1 (which has already been excited by light and lost an electron) to reduce it.
Step 5:
- Photosystem 1 has already been excited by absorbing light and losing electrons to its primary acceptor
- Electrons from the primary acceptor of photosystem 1 go through the second ETC to ferredoxin and NADP+ reductase to make NADPH (this NADPH is used in the Calvin cycle)