Photosynthesis Flashcards
What is photosynthesis?
it is a way of making glucose from light energy, water and CO2
- light energy is converted into chemical energy in form of glucose
define metabolic pathway
a series of small reactions controlled by enzymes
define photophosphorylation
light energy used to add phosphate to a molecule
define photolysis
light energy used to split a water molecule into protons, electrons and 02
Which coenzymes are used?
NADP which transfers Hydrogen
What is the structure of chloroplasts?
- they have a double membrane called chloroplast envelope
- thylakoids are stacked into structures called grana
- grana are linked together by parts of thylakoid membrane called lamellae
- contain photosynthetic pigments
Name the photosynthetic pigments and what are they
- coloured substances which absorbed light for photosynthesis
chlorophyll a
chlorophyll b
carotene
Where are photosynthetic pigments found?
In the thylakoid membrane attached to proteins
What is a photosystem?
protein attached to photosynthetic pigment
What are the 2 types of photosynthetic pigments?
primary and accessory
What are primary photosynthetic pigments?
- they are reaction centres
- electrons are excited during light dependent reaction
What are accessory photosynthetic pigments?
- make up light harvesting systems
- surround reaction centres
- transfer light energy to them to increase energy available to make electrons excited
How many photosystems are there and what are they used for?
2 (I AND II)
they are used to capture light energy
PSI absorbs light best at wavelength of 700nm
PSII absorbs light best at wavelength of 680nm
Where are the stroma?
- in the inner membrane of chloroplast
- surround thylakoids is stroma
- contains DNA, enzymes, organic acids and sugar
Where is the DNA?
in stroma
circular
multiple copies in each chloroplast
What else is stored in stroma?
carbohydrates that are produced but not used straight away are stored as starch grains in stroma
What are the 2 stages of photosynthesis?
- light-dependent reaction
- light-independent reaction
Explain what happens in light dependent reaction?
- occurs in thylakoids
- requires light
- absorbed by photosynthetic pigments in photosystems and it is converted into chemical energy
What are the 2 types of phosphorylation in light dependent reaction?
non cyclic and cyclic
both have different products
What do photosystems use the absorbed light energy for?
- making ATP from ADP and P (photophosphorylation
- making reduced NADP from NADP
- Photolysis - splitting of H20 into electrons, protons and 02
Explain what happens in noncyclic photophosphorylation
- photosystems are linked by electron carriers
- photosystems and electron carriers form electron transport chain
stage 1 - light energy is absorbed by PSII which is excites electrons in chlorophyll
- electrons move to a high energy level so have more energy
- high energy electrons move along transport chain to PSI
stage 2 - electrons need replacing as they leave PSII so photolysis occurs
stage 3 - excited electrons lose energy as they move along the chain
- energy used to move protons across membrane into thylakoid via proton pumps
- thylakoids have higher proton conc so form proton gradient
- protons move down concentration gradient into stroma via ATP synthase
- energy from movements combines ADP to P forming ATP
stage 4 - light energy absorbed by PSI which excites electrons to an even higher level
- electrons transferred to NADP along with proton from stroma forming reduced NADP
Explain cyclic photophosphorylation
- only uses PSI
- cyclic as electrons from chlorophyll aren’t passed to NADP but back to PSI via electron carriers
- no reduced NADP or O2
- produces small amounts of ATP
Explain light independent reaction or calvin cycle
- occurs in stoma
- CO2 fixation is where C from CO2 is fixed into organic molecule
- needs ATP and H ions to keep going
- CO2 enters leaf via stomata and diffuses into stroma
- combines RuBP (5C) forming 6C compound
- unstable so quickly breaks into 2 molecules of GP (3C)
- RuBisCo catalyses reaction between CO2 and RuBP
- 2 ATP from dependent reaction provides energy to turn GP (3C) into TP (3C)
- requires h ions from 2 reduced NADP from dependent reaction so NADP regenerated for next use
- TP converted into useful organic compounds such as glucose
cycle happens 6 times