photosynthesis (4.1, 4.2, 5.1, 5.2, 5.4, 5.6) (in depth) Flashcards

1
Q

What are the two stages of photosynthesis?

A

Light-dependent reactions and light-independent reactions (Calvin cycle)

The light-dependent reactions capture light energy, while the Calvin cycle uses that energy to fix CO2 into sugar.

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2
Q

What occurs during the light-dependent reactions?

A

Direct absorption of photons, synthesis of NADPH and ATP, splitting of water

Water molecules are split to provide electrons, establishing a proton gradient.

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3
Q

What is the primary function of ATP in the Calvin cycle?

A

To provide energy for converting CO2 into sugar

ATP is generated during light-dependent reactions and used in the Calvin cycle.

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4
Q

What is produced from CO2 during the Calvin cycle?

A

3-carbon carbohydrate called G3P

G3P can be used to form larger molecules such as glucose.

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5
Q

Where do the light-dependent and independent reactions take place?

A

In the chloroplasts

Chloroplasts are organelles with three membranes: outer membrane, inner membrane, and thylakoid membrane.

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6
Q

What is the role of pigment molecules in photosynthesis?

A

To absorb light energy and transfer it to chlorophyll

Pigment molecules include chlorophylls and carotenoids.

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7
Q

What are the three outcomes for an excited-state electron in a pigment molecule?

A
  • Returns to ground state and releases energy as thermal energy or fluorescence
  • Transfers energy to a different electron
  • Accepted by a primary acceptor and becomes reduced

Fluorescence emits lower energy light than absorbed.

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8
Q

What are the primary photosynthetic pigments?

A

Chlorophyll a and chlorophyll b

Carotenoids are accessory pigments that help transfer energy to chlorophyll a.

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9
Q

What is the absorption spectrum of chlorophyll a?

A

Strongly absorbs blue and red light but does not absorb green and yellow light

This characteristic affects the efficiency of photosynthesis.

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10
Q

What happens in photosystem II?

A

Splitting of water into electrons, protons, and oxygen gas

Energy absorbed from light excites electrons in P680 chlorophyll.

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11
Q

What is the function of the primary electron acceptor?

A

Accepts electrons from excited chlorophyll and becomes reduced

This initiates the electron transport chain.

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12
Q

What is linear electron transport?

A

Transfer of high energy electrons through a series of proteins, generating ATP and NADPH

Involves photosystems I and II.

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13
Q

What is chemiosmotic synthesis of ATP?

A

Creation of ATP using a proton gradient across the thylakoid membrane

High concentration of protons in lumen drives ATP synthase.

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14
Q

What is carbon fixation?

A

Conversion of inorganic CO2 into organic sugar

This process is facilitated by the enzyme rubisco.

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15
Q

What are the three phases of the Calvin cycle?

A
  • Fixation
  • Reduction
  • Regeneration

Each phase plays a role in synthesizing glucose.

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16
Q

What is produced during the reduction phase of the Calvin cycle?

A

Glyceraldehyde-3-phosphate (G3P)

This occurs after 3-phosphoglycerate receives a phosphate and is reduced by NADPH.

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17
Q

What is the role of light energy in photosynthesis?

A

Excites electrons, allowing them to move from low energy (water) to high energy (NADPH)

This process is crucial for the conversion of light energy into chemical energy.

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18
Q

What is cyclic electron transport?

A

Electrons are donated back to plastoquinone, producing more ATP

This process does not produce NADPH and contributes to the proton gradient.

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19
Q

what is aerobic cellular respiration? and how does it work?

A

a cellular respiration process using oxygen to harvest energy from organic compounds. it breaks down glucose with oxygen and water and CO2

20
Q

what are obligate aerobes?

A

organisms that can’t live without O2 so they use cellular respiration

21
Q

what is substrate level phosphorylation?

A

formation of ATP by a direct transfer of Pi from a substrate

22
Q

what is oxidative phosphorylation?

A

it forms ATP by indirect energy transfers from redox reactions

23
Q

what are the four steps of cellular respiration?

A

glycolysis
pyruvate oxidation
citric acid cycle
electron transport chain

24
Q

what is glycolysis in cellular respiration

A

happens in the cytosol and breaks down two carbon molecules of pyruvate (ATP and NADH byproduct)

25
what is pyruvate oxidation in cellular respiration
happens in mitochondria each pyruvate is oxidized by NAD+ and two acetyl-CoA is byproduct
26
what is the citric acid cycle in cellular respiration
acetyl-CoA is oxidized to CO2, and NADH and FADH2 is formed
27
what is the electron transport chain and oxidative phosphorylation in cellular respiration
NADH and FADH2 molecules are oxidized, its electrons go to O2 and makes water. energy is used to make ATP
28
what is the mitochondria composed of
inner and outer membrane, space between is called the inner membrane space
29
what is an anaerobic pathway
a way that cells make energy without oxygen
30
what is fermentation
uses organic compounds as a final oxidizing agent to produce energy
31
what are obligate aerobes
can't survive when O2 is present, relies on anaerobic pathways for energy
32
what is glycolysis
ten step breaking down of things to release energy that happens in the cytosol. glucose is split in half to make pyruvate.
33
list the two phases of glycolysis
energy investment phase (first five steps) and energy payoff phase (last five steps)
34
list out glycolysis
glucose glucose-6-phosphate fructose-6-phosphate fructose-1, 6-biphosphate DHAP and G3P 1, 3-biphosphoglycerate 3-phosphoglycerate 2-phosphoglycerate phosphoenolpyruvate (PEP) pyruvate
35
where does pyruvate oxidation occur
the mitochondria
36
what happens to acetyl-CoA in pyruvate oxidation
decarboxylated, dehydrogenated, electron transfer, add coenzyme A
37
what happens in the citric acid cycle
glucose’s carbons are oxidized into CO2 and H+ are taken by coenzymes
38
how many times does the krebs cycle occur and why
it occurs twice because two acetyl-CoA’s are made from pyruvate oxidation
39
what is the acronym for the krebs cycle
Oscar Can’t Imagine Killing Sam Smith For Money
40
what are the steps of the krebs cycle
Oxaloacetate + acetyl-CoA Citrate Isocitrtate alpha-Ketogluterate Succinyl Succinate Fumerate Malate
41
what happens in the electron transport chain
reduced coenzymes are oxidized electrons make a hydrogen-ion gradient across a membrane electron acceptor is reduced by O2, ATP produced
42
where does the electron transport chain occur
the mitochondria’s inner membrane
43
how does the electron transport chain transfer hydrogen’s electrons
redox reactions
44
what are the two characteristics of the electron transport chain
- highly exergonic (gets more and more stable) - each molecule down the chain gets more electronegative
45
what is chemiosmosis
a process where ATP is synthesized using energy from an electrochemical gradient and ATP synthase
46
what happens because of chemiosmosis?
- protons accumulate in the inter membrane and create a proton gradient - proton motive force combines a chemical and electrical gradient
47
what comes in and out of the krebs cycle
acetyl-CoA + **oxaloacetate** H2O comes in and out **isocitrate** NAD comes in, NADH and CO2 comes out **alpha-ketogluterate** NAD comes in, NADH comes out **succinyl** GTP comes in, GDP and COA comes out **succinate** FAD comes in, FADH comes out **fumerate** H2O comes in **Malate**