photosynthesis Flashcards
what is an organic compound?
a compound that contains carbon e.g CO2/ lipids/ manure
what is an inorganic compound?
a compound that does noy contain carbon e.g phosphate
redux meaning
oxidation and reduction
what are co-enzymes?
non protein molecules that help enzymes to work by transferring other molecules around
phosphorylation meaning
adding a phosphate (ADP + Pi = ATP)
photo- phosphorylation meaning
using light energy to add a phosphate
what is photolysis?
using light energy to split a water molecule
what is an electron transport chain?
when electrons flow through proteins (electron carriers) in a membrane
proton meaning
a hydrogen ion (H+)
what is the light dependent reaction?
uses light energy to make ATP and NADP
takes place on the membrane of the thylakoid (large SA)
sometimes called photo phosphorylation
what is the light independent reaction?
uses the products of LDR (ATP AND NADP) to make useful organic compounds
takes place in the stroma
also called the calvin cycle
wheee does photosynthesis take place?
the leaf is the main photosynthetic structure in eukaryotic plants, chloroplasts are the cellular organelles within the leaf where photosynthesis takes place
the chloroplast…
absorbs light energy which is used for photosynthesis to make useful organic compounds
the thylakoid…
has a high surface area to increase the rate of photosynthesis
contains photosynthetic pigments (chlorophyll a chlorophyll b and carotene)
some have tubular extensions called inter-granas lamellae which join to thylakoids in adjacent grana
structure of a chloroplast
grana are stacks of thylakoids (LDR)
the stroke is a floyd filled matrix (LIR)
(starch grains and chloroplast dna)
what are the raw materials of photosynthesis
water carbon dioxide and light
what are the products of photosynthesis?
oxygen and glucose
how are leaves adapted for photosynthesis?
- large SA absorbs sunlight
- arrangement of leaves that avoids overlapping/ shadowing of leaves
- thin (light absorbed within first few micrometers and short gas diffusion distance)
- transparent cuticle and epidermis that let light through to the photosynthetic mesophyll cells beneath
- long narrow upper mesophyll cells packed with chloroplast that collect sunlight
- numerous stomata for gas exchange mesophyll cells only a short diffusion pathway for one another
- stomata that open/close in response to light
- many air specs in lower mesophyll rapid diffusion of gases (o2 and co2)
- network of xylem and phloem
stages of the LDR
photo phosphorylation: occurs in the membrane of thylakoids
1. photosynthetic pigments (chlorophyll) absorb light energy
2. this excites an electron which leaves the chlorophyll molecule (in PS2) so chlorophyll becomes ionised (photoionisation)
3. the electrons that leave the chlorophyll are taken up by a molecule called the electron carrier. chlorophyll oxidised. electron carrier reduced.
4. electron moves along the electron transport chain by electron carriers in a series of oxidation- reduction reactions
5. the electron releases energy as each carrier is at a slightly lower energy level
6. this energy is used to join ADP + Pi to make ATP
7. NADP is reduced to form reduced NADP (NADPH)
8. photolysis of water makes protons (H+) electrons (e-) and oxygen
what is the chemiosmotic theory (ATP)
- each thylakoid is in an enclosed chamber into which protons (H+) are pumped from the strong using protein carriers in the thylakoid membrane called proton pumps
- the energy comes from when electrons are released in photolysis which also produces protons increasing their concentration inside the thylakoid space
- this creates and maintains a concentration gradient of protons across the thylakoid membrane (high in thylakoid space, low in stroma)
- protons cross the thylakoid membrane through ATP synthase channel proteins. the rest is impermeable. these channels form small granules so are known as stalked granules
- as protons pass through ATP synthase channels they cause changes to the structure of enzymes which then catalysis the combination of ADP with inorganic phosphate to form ATP
what is photolysis
the loss of electrons when light strikes a chlorophyll molecule leaves it short of electrons. if chlorophyll is to continue absorbing light energy these must be replaced. These are provider from water molecules that are split using light energy. this also yields protons.
what happens to the protons yielded in photolysis of water
they pass out of the thylakoid space through ATP synthase channels and are taken up by an electron carrier (NADP). the NADP becomes reduced. this is the main product of the LDR stage and enters the LIR stage taking with it the electrons from the chlorophyll molecules . NADPH is a potential source of chemical energy to the plant
photolysis equation
2H2O —— 4H+ + 4e- + O2
water ——— protons + electrons + oxygen
adaptations of chloroplasts for photosynthesis
thylakoid membrane large SA
network of proteins in the grana hold the chlorophyll in a way that allows maximum absorption of light
granal membranes have ATP synthase channels that catalyse the production of light. semi permeable to establish a proton gradient
contain DNA and ribosomes so can manufacture proteins involved in LDR