Photo Synthesissisis Brooo Flashcards
Photosynthesis is, evolved
Light energy converted into
Why redox bro
Is also an ( reaction
A physiochemical or photo biochemical in which organic compounds ( glucose) are synthesised from inorganic raw material ( h2o,co2) in presence of light , O2
Chemical energy
Oxidation of h2 ( provided h+ and e ) during light reaction reduction of co2 ( accepts h+ &e ) during dark reaction
Anabolic ( synthesing ) & endergonic ( energy absorbing ) process
Variegated leaf experiment explain
On testing
Molls half leaf experiment
Conclusion
Leaf that was partially covered with black paper and one was exposed to light
Photosynthesis occurred only in green parts of leaves in presence of light
Part of leaf is enclosed in test tube containing some KOH soaked cotton which absorbs co2 while the other half is exposed to air
Shows that co2 is required for photosynthesis
Joseph Priestley (year), plant , conclusion
Jan Ingenhousz ( year) same setup but , showed
Also showed that
1770 , ,mint , plants restore to air whatever breathing animals and burning candles remove
1730-1799) in dark showed that sunlight is essential to plant process that somehow purifies the air
It is only the green part of the plants that could release oxygen
J.V. Sachs year, provided evidence And it is stored as
Later studies showed that
Van Niel was , used showed that
Conclusion
1854 , for production of glucose when plants grow , as starch
The green substance in plants is located in special bodies within plant cells
Microbiologist , purple and green bacteria , photosynthesis is light dependent reaction in which hydrogen from a suitable oxidisable compound reduce co2 to carbohydrate
O2 evolved by green plant comes from h2o not from co2
Van niel reaction
Ruben and Kamen year
2H2A + co2 = (light ) = 2A + CH2O + H2O ( H2A = suitable oxidisable compound or H- donar
1941 , used o18 (radioisotopic techniques) to show experimentally that o2 in photosynthesis released from water
Photosynthesis take place
Within chloroplast there is
Membranous system responsible for ( reaction
Stroma
In green leaves of plants , also in other green parts of plant
Membranous system consisting of grana , the stroma lamellae , matrix stroma
( thylakoid ) = trapping the light energy, for synthesis of ATP and nadph ( light ,photochemical
Enzymatic reactions synthesis sugar , which in turns form starch ( dark , carbon
Usually chloroplast align themselves
Parastrophe
epistrophe
Apostrophe
Photosynthetic pigments are
Along the walls of mesophyll cells
High intensity light,parallel to incident light /. Lateral walls
Low light intensity, perpendicular to incident light
Moderate light intensity ( random
Special molecules those absorb , transmit,reflect different colours of light from visible spectrum of sunlight
Pigments appear,uses
Photosynthetic pigment
Leaf pigment can be separated ,result
chlorophyll colour,required,solvents
In colour which it reflects, uses colour it absorbs
Chlorophyll,carotenoids,phycobilins
Through paper chromatography, chromatogram
Green,light is required for synthesis,soluble in organic solvents
Chl a , found, colour
Chl b
Structure of chlorophyll is
Head size , it is , character
Tail
Universal pigment , blue green
Euglenoids,green algae,higher plants ,yellow green
Tadpole like
Porphyrin , 15x15 A, hydrophilic, tetrapyrole structure with mg +nt in centre
Phytol (C20H39OH ) , 20 A, hydrophobic, remain embedded in lipid bilayer of thylakoids membrane
Chlorophyll a
Chlorophyll b
Chlorophyll synthesis
Catalysed
Carotenes , colour, contains
Xanthphyll
C55H77O5N4Mg , ch3 group in pyrrol ring
C55H70O6N4Mg , CHO group in pyrrol ring
Succinyl CoA + glycine = protochlorophyll = chlorophyll ( light , 2H)
Iron fe
Yellow orange , carbon and hydrogen
Yellow , carbon and hydrogen and oxygen
Common in plants
Fn of carotenoids
Phycobillins are soluble, tail
Types
Occur
Beta - carotene , lutein ( xanthophll )
Accessory pigments, protect chl a from photo oxidation
also protect by converting lethal nascent oxygen into unharmful molecular oxygen thus called shield pigments
Hot water soluble, mg and phytol tail
Phycocyanin = blue , phycoerythtin = red , allophycocyanin = light blue
In BGA and red algae
PAR
Absorption spectrum
Action spectrum y axis
Maximum
T.w engelman used
Photosynthesis active radiation = 400-700 nm
Chl a shows maximum absorption at blue light then red light
Rate of photosynthesis, measured by O2 released
Red then blue
Green algae chadophra , in suspection of aerobic bacteria
Light reaction / , include
Who worked (on)
Photo system
The LHC are made up of
Light absorption,water splitting,oxygen release,formation of high energy chemical intermediate like ATP and NaDPH
Emerson , Arnold worked on chlorella
The groups of photosynthesis pigments in thylakoids mem
200-400 molecules of pigments bound to proteins
Photo phosphorylation
Non cyclic/ ,
Includes
Pathway
Moment of electron is
Synthesis of atp from ADP and inorganic phosphate (ip) with the help of light energy
Z scheme and splitting of water
Both ps 1, ps2 , occurs only in stroma thylakoids
Ps 2 => pheophytin => pheoquinone => cytochrome B6F => plastocynin => ps 1
Is downhill in terms of oxidation o redox potential scale
Ps1 pathway
The splitting of water is associated with (located
Z scheme is formed
What not formed in cyclic
FRS to FD
PS2 , on inner side of membrane of thylakoids
When all the carries are placed in sequence on redox potential scale
No o2 evolved,nadph2 ( reducing power ) not formed
Only atp formed