PHARM 411-Biotechnology

Question 1

What is Biotechnology?

Answer 1

• A broad group of disciplines that exploit biological cells, biomolecular processes to develop new technologies
• Important of biotech continues to increase

Question 2

Objective: Recombinant

what is the structure of Insulin

Answer 2

• Composed of 2 different amino acid chains connected to disulfide bond
• Synthesized naturally as one aa called Preproinsulin
• Later bacteria were engineered to produced the Proinsulin

Question 3

Objective: Recombinant

Erythropoeitin

Answer 3

• Aumenting an existing pathway

- A glycoprotein hormon that controls RBCs production

Question 4

Which protein is the MOST successful biotec drug in history?

Answer 4

-Erythropoetin

Question 5

Function of Bevacizumab

Answer 5

-Binds VEGF-A keeping it from binding to VEGF receptor

Question 6

Function of Trastuzumab

Answer 6

-Binds HER neu cell surface receptor, STOPS signaling by HER2

Question 7

Function of Adalimumam*

Answer 7

-Binds TNFa to reduce the inflammatory response

Question 8

What is Etanercept used to treat and what is it bind?

Answer 8

• A recomminan FUSION protein used to treat an AUTOIMMUNE DISEASE
• Binds to TNFa and inhibits it

Question 9

What immunoglobin does Etanercept used to form byfusing TNFa receptor surface domains?

Answer 9

IgG1 Fc

Question 10

What are the steps to make Biological Drugs

Answer 10

1) Identify Gene
2. Isolate gene
3. Amplify the gene using PCR tec
4. Place the gene in a vector