pH and Buffers Flashcards
example of weak acid
acetic acid
Ka=
h+ x conjugate base/ weak acid
it tells us the degree of dissociation to determine if weak or strong acid
pH=
-log (H+)
pKa=
-log (Ka)
henderson- hasselbalch equation
pH= pka+log(conjugate base conc./ acid conc.)
define buffer
molecules that RESIST pH changes by the small additions of acid or base to a system
- usually mixture of weak acid/ its conjugate base
OR weak base and its conjugate acid
midpoint in titration tells us..
WHERE the buffer system buffers
- can extend 1pH unit either side of the pKa value
what is the midpoint
point where pH=pKa
when the conjugate base concentration is higher then this buffer is good at buffering….
added acid
when the acid concentration is higher then this buffer is good at buffering…
added bases
how is INTRA-cellular pH maintained at 7.4
phosphate and histidine side chain of proteins
how is EXTRA-cellular pH maintained at 7.4
the BICARBONATE buffer system
name the 3 buffer system is saliva
- bicarbonate - most important in stimulated saliva
- phosphate
- proteins - histidine chains