Peptide Synthesis Flashcards
1
Q
SPPS can synthesize peptides up to _ aa long
A
50
2
Q
If you need a long peptide, what is an approach you could take?
A
Convergent synthesis strategies
3
Q
Advantage of chem synthesis of peptides over recombinant approaches: (2)
A
- Non natural modifications possible
- Precise incorporation of translational modifications
4
Q
Disadvantages of chem synthesis of peptides
A
Limited peptide length
5
Q
Mention approaches of peptide ligation (7)
A
- prior-thiol-capture strategy
- thiazolidine ligation
- native chemical ligation
- synthesis of peptide thioesters
- Traceless Staudinger ligation
- peptide phosphinothioesters
- protein semisynthesis
6
Q
What strategies give native peptide bonds?
A
- prior-thiol-capture strategy
- native chemical ligation
7
Q
Why is cys and not met used for peptide ligation?
A
Because cys is able to perform a nucleophilic attack (has a thiol group) and met not (has thioether group)
8
Q
If you don’t want S atoms in your peptide at the site of linkage, what can you do? (2)
A
- Desulfurization
-Auxiliary mediated ligation
9
Q
What kind of auxiliaries are there for peptide ligation? (3)
A
- Acid sensitive
- Photosensitive
- Base sensitive
