PCR Application Flashcards
1
Q
What is PCR used for?
A
-Routine diagnostic tool used for identification, confirmation and quantification of specific DNA sequence
2
Q
Examples of PCR application
A
- Presence or absence of diseases such as TB
- Allow Differentiating between closely related organisms for example “swine flu vs human influenza” both H1N1 subtypes
- PCR may also allow us to determine how much is there for example measuring bacterial or viral load
3
Q
What are the two diagnostic methods in PCR called?
A
-Real-time PCR or quantitative PCR
4
Q
What is the purpose of fluorescence?
A
