PCR Flashcards
1
Q
What PCR?
A
A biochemical process capable of amplifying a single DNA molecule into millions of copies in a short time
2
Q
What are the three steps of PCR?
A
Denaturation
Annealing
Extension
3
Q
Describe the denaturation stage of PCR?
A
dsDNA templates heated to separate the strands
4
Q
Describe the annealing phase of PCR
A
Short DNA molecules (primers) bind to flanking regions of the target DNA
5
Q
Describe the extension phase of PCR
A
DNA polymerase extends the 3’ end of each primer along the template strands
6
Q
At which temperature is PCR performed?
A
Stage 1 - 95
Stage 2 - 61
Stage 3 - 72
7
Q
What are the clinical applications of PCR?
A
Fingerprinting
Genetic diagnosis
Viral detection
8
Q
What is in the mastermix?
A
dNTP
MgCl
Reaction buffer
Taq polymerase
