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Infection and Immunity > PCR > Flashcards

PCR Flashcards

1
Q

What is PCR

A

Enables copying and amplification of small, specific regions of DNA (50bp to 50Kb)

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2
Q

STEPS OF PCR

A

Denaturing (95C)
Annealing (50-60C)
Elongation (72C)

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3
Q

Taq polymerase

A

These are extremophiles. Optimal temperatures for Taq is 72degrees. It has no proof reading ability.

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Infection and Immunity (3 decks)

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