PCR Flashcards
1
Q
What is PCR’s purpose?
A
to make billions of copies of DNA
2
Q
PCR allows scientists to target ______ regions of the DNA
A
specific
3
Q
PCR needs _______ to target specific regions and cut DNA
A
Primer
4
Q
What makes up the “Master Mix?”
A
Nucleotides (dNTPs)
Buffer solution
Taq polymerase
Primers
5
Q
What are the steps of PCR?
A
Denaturation, annealing, elongation
6
Q
What is Taq polymerase?
A
enzyme
7
Q
What happens during step 1, denaturation?
A
DNA strands dissociate into single strands after being heated 95°C
8
Q
What happens during step 2, annealing?
A
The DNA strands are cooled 45-70°C and primers bind to them
