PCR Flashcards
1
Q
Who invented PCR?
A
Kary Mullis
2
Q
What are the steps of PCR?
A
- Primers
- Heat (93°C)
- Anneal (50-60°C)
- Extend (72°C)
3
Q
How many cycles does a PCR typically involve?
A
25-30
4
Q
What are the applications of PCR?
A
- Cloning
- DNA quantification
- Diagnostics
- DNA sequencing
- RT-PCR
- DNA fingerprinting
