PCR Flashcards
1
Q
what is PCR used for
A
To make copies of an exact region of DNA ( not the entire genome)
2
Q
What does the 5’ end of DNA always have
A
a phosphate group
3
Q
what do the 3’ ends of DNA always have
A
hydroxyl group
4
Q
Necessary reagents
A
DNA, Target DNA, Primers, dNTPs, DNA polymerase
5
Q
what are the three key steps
A
Denaturation, Annealing, Extension
6
Q
what is denaturation and what happens
A
breaks the weak linkages or bonds (eg, hydrogen bonds)
7
Q
what is annealing
A
temperature is lowered to enable;e the DNA primers to attach to the template DNA
8
Q
what is extension
A
extension of the primer, a copy of a DNA strand is formed in this step
