PBS AND COUNTING Flashcards
- provide valuable information regarding px health
pbs and counting
preferred edta tube
liquid tripotassium edta
evacuated tubes used in collection
anticoagulant-free blood
lavender topped tubes (containing dipotassium or tripotassium edta)
capstone of a panel of test is called
cbc or histogram
t/f: after collection, it must be made within 24 hours of drawing the specimen
f - dapat 4 hrs lang
- in vitro phenomenon
- platelets adhere to neutrophils
- causes pseudothrombocytopenia in automated methods
platelet satellitosis
result of platelet satellitosis in PLT and WBC count
low platelet count
false increase wbc count
what tube is used to correct plt satellitosis
sodium citrate tube
ratio of sodium citrate tube
9:1
made immediately at the px’s side
disadvantages: plt clumping, few films can be made directly
finger and heel punctures
easiest to master
most convenient and commonly method used
manual wedge method
size of glass slide, drop of blood and angle
glass slide: 3x1 inch
blood: 2-3mm in dm
angle: 30-45 degrees
makes the cell more visible and to allow their morphology to be evaluated
staining
stains pbs and bone marrow smears
pure wright stain/ giemsa-wright stain (romanowsky stain)
dye used in polychrome (wright and leishman) stain
eosin & methylene blue
dye used in giemsa stain
eosin, azure b, methylene blue
fast and easy
the whole process takes about 1 minute
quick stain
used as buffer in quick stain
aged distilled water
- performed over a sink or pan with a staining rack
- strains: filtered before use or poured from the bottle
manual technique
in manual technique, stains must be left on the slide for how many minutes
1-3 minutes
this is an indicator that the mixing is correct
metallic sheen or green scum
t/f: best results are obtained on fresh slides
t
rbc appearance after staining
appears orange to salmon pink
neutrophil appearance after staining
cytoplasm should be pink to tan with violet or lilac granules
wbc appearance after staining
nuclei turned purple to blue
eosinophil appearance after staining
bright orange refractile granules
ideal area of viewing in pbs
between the feathered edge and thick region
performed in a systematic manner
differential counting
this pattern is preferred because it minimizes distribution errors and ensures that each cell is counted once
back-and-forth serpentine or battlement track pattern
push-down counters or newer computer-interfaced key pads
counters
t/f: results are reported as tenths
f -percentage
