pbr322 Flashcards
Describe the nomenclature of PBR322.
What is the significance of ORI ?
List out the two most important features of PBR322.
How many restriction sites are in PBR322 ?
How does PBR322 work as a cloning vector ?
List out the selectable markers in PBR322
Write the use of PBR322.
What is PBR322 plasmid ?
Define PSC101.
How is Ti plasmid used in Genetic Engineering ?
What is the function of Ti Plasmid ?
Sketch out the Ti plasmid vector
Mention the use of Ti plasmid in Genetic Engineering.
Why are bacteriophage used as vectors ?
What are bacteriophage vectors ? Name two commonly used phage vectors
Why are Plasmids and bacteriophages used as cloning vector ?
How can bacteriophage be used to clone a gene ?
State the advantages of lambda phage vector.
Define M13 Phage vector.
Denote the structure and function of bacteriophage.
Define lambda phage vector
Define cosmid Vector.
Differentiate between plasmid and cosmid.
Write the advantages of the use of a cosmid vector.
Define YAC.
Define BAC.
Differentiate between YAC and BAC.
Write the component’s of Yeast artificial chromosome.
How cosmids are constructed ?
Define PJB8 vector.
Define PYAC3 vector.
Write the full form of ATCC abd MTCC.
What is DNA ?
What is RNA ?
Define Gene.
Define Genome.
Define gene cloning.
Define rDNA Technology.
How is a recombinant DNA produced?
Define gene Therapy.
Define genomic library.
Discuss the principle of genetic engineering.
Identify the applications of rDNA technology in agriculture.
Which method is used for strain improvement ?
How can microbial strain be improved ?
Write the characteristics of strain improvement.
Which technology has a great application in strain improvement.
What are the main characteristics of industrial strains ?
How microbes are useful in industrial production ?
List out the common industrial product of microbes.
Write the difference between mutation and rDNA technology.
How can recombinant DNA be used in industry ?
What are the useful products of recombinant DNA ?
List out the pharmaceutical products of recombinant DNA.
How is genetic engineering used in industrial biotechnology ?
How recombinant DNA technology is used in medicine ?
How is insulin produced by rDNA technology ?
What is the function of restriction enzyme in rDNA Technology ?
Define vector
List the types of vector
Identify the role of plasmids in Genetic Engineering.
Name commonly used vectors in Genetic Engineering.
Differentiate between pathogens and vectors
How do cloning vectors work ?
List out the three main components of a cloning vector.
Why are vectors important in cloning?
How is DNA inserted into a cloning vector ?
Why plasmid is used as a cloning vector ?
List the different types of cloning vectors.
Define cloning vector.
Define expression vector.
Distinguish between cloning vector and expression vector
Who discovered plasmid in biotechnology ?
Write the importance of plasmid in biotechnology.
Why are plasmids essential for rDNA technology?
Define Genetic Engineering.
Define PSC101.
