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cell biology > pbr322 > Flashcards

pbr322 Flashcards

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1
Q

Describe the nomenclature of PBR322.

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2
Q

What is the significance of ORI ?

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3
Q

List out the two most important features of PBR322.

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4
Q

How many restriction sites are in PBR322 ?

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5
Q

How does PBR322 work as a cloning vector ?

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6
Q

List out the selectable markers in PBR322

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7
Q

Write the use of PBR322.

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8
Q

What is PBR322 plasmid ?

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9
Q

Define PSC101.

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10
Q

How is Ti plasmid used in Genetic Engineering ?

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11
Q

What is the function of Ti Plasmid ?

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12
Q

Sketch out the Ti plasmid vector

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13
Q

Mention the use of Ti plasmid in Genetic Engineering.

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14
Q

Why are bacteriophage used as vectors ?

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15
Q

What are bacteriophage vectors ? Name two commonly used phage vectors

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16
Q

Why are Plasmids and bacteriophages used as cloning vector ?

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17
Q

How can bacteriophage be used to clone a gene ?

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18
Q

State the advantages of lambda phage vector.

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19
Q

Define M13 Phage vector.

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20
Q

Denote the structure and function of bacteriophage.

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21
Q

Define lambda phage vector

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22
Q

Define cosmid Vector.

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23
Q

Differentiate between plasmid and cosmid.

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24
Q

Write the advantages of the use of a cosmid vector.

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25
Q

Define YAC.

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26
Q

Define BAC.

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27
Q

Differentiate between YAC and BAC.

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28
Q

Write the component’s of Yeast artificial chromosome.

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29
Q

How cosmids are constructed ?

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30
Q

Define PJB8 vector.

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31
Q

Define PYAC3 vector.

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32
Q

Write the full form of ATCC abd MTCC.

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33
Q

What is DNA ?

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34
Q

What is RNA ?

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35
Q

Define Gene.

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36
Q

Define Genome.

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37
Q

Define gene cloning.

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38
Q

Define rDNA Technology.

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39
Q

How is a recombinant DNA produced?

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40
Q

Define gene Therapy.

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41
Q

Define genomic library.

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42
Q

Discuss the principle of genetic engineering.

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43
Q

Identify the applications of rDNA technology in agriculture.

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44
Q

Which method is used for strain improvement ?

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45
Q

How can microbial strain be improved ?

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46
Q

Write the characteristics of strain improvement.

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47
Q

Which technology has a great application in strain improvement.

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48
Q

What are the main characteristics of industrial strains ?

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49
Q

How microbes are useful in industrial production ?

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50
Q

List out the common industrial product of microbes.

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51
Q

Write the difference between mutation and rDNA technology.

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52
Q

How can recombinant DNA be used in industry ?

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53
Q

What are the useful products of recombinant DNA ?

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54
Q

List out the pharmaceutical products of recombinant DNA.

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55
Q

How is genetic engineering used in industrial biotechnology ?

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56
Q

How recombinant DNA technology is used in medicine ?

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57
Q

How is insulin produced by rDNA technology ?

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58
Q

What is the function of restriction enzyme in rDNA Technology ?

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59
Q

Define vector

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60
Q

List the types of vector

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61
Q

Identify the role of plasmids in Genetic Engineering.

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62
Q

Name commonly used vectors in Genetic Engineering.

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63
Q

Differentiate between pathogens and vectors

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64
Q

How do cloning vectors work ?

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65
Q

List out the three main components of a cloning vector.

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66
Q

Why are vectors important in cloning?

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67
Q

How is DNA inserted into a cloning vector ?

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68
Q

Why plasmid is used as a cloning vector ?

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69
Q

List the different types of cloning vectors.

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70
Q

Define cloning vector.

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71
Q

Define expression vector.

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72
Q

Distinguish between cloning vector and expression vector

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73
Q

Who discovered plasmid in biotechnology ?

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74
Q

Write the importance of plasmid in biotechnology.

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75
Q

Why are plasmids essential for rDNA technology?

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76
Q

Define Genetic Engineering.

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77
Q

Define PSC101.

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