Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

HISTOTECH > Pathology > Flashcards

Pathology Flashcards

1
Q

What drug can cause Gray Baby Syndrome and aplastic anemia?

A

Chloramphenicol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Surgical connection between two structures

A

Anastomosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Bevel angle

A

27-32 degrees

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Record of BB donor/recipient records

A

Indefinitely

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Surgical pathology reports

A

10 yrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Cytogenetic reports

A

20 yrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Cytogenetics diagnostic images record

A

20 yrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Surgical Pathology and Cytology

Releasing of Results

A

Within 24 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Frozen Section

Releasing of results

A

5-15 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Autopsy Report

Releasing of Results

A

1 week

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Copies of Report

A

1 Doctor
1 Patient
1 File

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Processing of Tissues

FDDCIETSSML (11 steps)

A 
F Fixation
D Decalcification
D Dehydration
C Clearing / Dealcoholization
 I  Impregnation/ Infiltration
 E Embedding / Casting / Blocking
 T Trimming
 S Sectioning/ Microtomy
 S Staining
 M Mounting
 L Labeling
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Primary purpose of Fixation

A

Preserve the morphological and chemical integrity of the cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Secondary purpose of fixation

A

Harden and protect the tissue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Most important reaction of fixation

A

Stabilization of proteins by cross linking

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Electron Microscopy Temperature

A

0-4 deg Celsius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Speed of fixation

A

1 mm per hr

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Volume

A

10-20:1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Reference Lab of Histopath

A

National Kidney Transplant Institute (NKTI)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

8 examples of Microanatomical Fixatives

A
  • 10% NBF
  • 10% Formol Saline
  • Formol Sublimate
  • Zenker’s Formol
  • Zenker’s
  • Heidenhein’s Susa
  • Bouin’s
  • Brasil’s
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Nuclear Fixative char.

And examples

A 
(+) glacial acetic acid
<4.6 pH
20-22 deg Celsius
BeNCH Fix
B - Bouin’s
N - Newcomer’s
C - Carnoy’s Fluid
H - Heidenhein’s

Fix - Flemming’s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Cytoplasmic Fixative char

And examples

A

pH >4.6
(-) glacial HAc

ROHFFour
R - Regaud’s
O - Orth’s
H - Helly’a
F - Flemming’s w/o HAc
Four - Formalin with Post Chromatization
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Alcoholic formalin (Gendre’s) purpose

A

Mucus containing specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Another name for Heidenhain’s Susa

A

Acid Mercuric Chloride

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Another name for Regaud’s Fluid And purpose

A

Muller’s

-chromatin, mitochondria, golgi bodies, colloid containing tissue (thyroid)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Metallic fixatives (3 classes)

A

Mercuric Chloride
Chromate Fixative
Lead Fixative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Mercuric Chloride Fixatives (7)

A 
Zenker’s Fluid
Zenker’s Formol (Helly’s)
Heidenhain’s Susa
B-5 Fixative
Ohlmacher’s Fluid
Schaudinn’s Fluid
Carnoy-Lebrum solution
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Chromate Fixative (4)

A

Chromic acid
Potassium dichromate
Regaud’s fluid (Muller’s)
Orth’s fluid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

How to remove Picric Acid yellow color

And 2 examples

A

70% ethanol + 5% sodium thiosulfate

Bouin’s solution
Brasil’s Alcoholic Picroformol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

At what temperature does Glacial Acetic Acid solidify

A

17 deg celsius

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Alcohol Fixatives (6)

A 
Methanol
Isopropyl Alcohol
Ethanol
Carnoy’s fluid
Newcomer’s fluid
Alcoholic formalin
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Osmium tetroxide / Osmic Acid

Char and examples

A

Inhibits hematoxylin
Causes conjunctivitis or blindness
Kept in dark colored storage (reduction by sunlight prevention)

Flemming’s with or without HAc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Acetone purpose

A

For water diffusible enzyme studies and brain studies (e.g. rabies)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Fixatives for Electron Microscopy

A 
Glutaraldehyde
Platinic Chloride
Platinic Chloride formalin
Gold Chloride
Osmium tetroxide (osmium tetroxide)
10% NBF
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

How to remove Chromate pigment

A

Acid-alcohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

How to remove black precipitate crystals?

A

Cold water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

Hydrochloric Acid example and purpose

A

Von Ebner’s Fluid - teeth and small pieces of bones

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

Fixative and Decalcifying Agent

A

5% Formic Acid
Trichloroacetic Acid
Chromic Acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

Tissue Softeners

A

4% aqueous phenol
Molliflex
2% HCl
1% HCl in 70% Alcohol

40
Q

Inaccurate test to measure completeness of decalcification

A

Physical or Mechanical

41
Q

Most accurate and reliable but expensive test to measure completeness of decalcification

42
Q

Most commonly used technique to measure completeness of decalcification

A

Chemical Method (calcium oxalate test)

43
Q

Ratio of dehydrating agent and tissue

44
Q

Plants and animals microtechnique dehydrating agent

45
Q

Another name for cellosolve (dehydrating agent)

A

Ethylene glycol monoethyl ether

46
Q

Another name for dioxane (dehydrating agent)

A

Diethylene dioxide

47
Q

Both dehydrating and clearing agent

A

Diethylene dioxide

Tetrahydrofuran

48
Q

Requirements of a clearing agent

A
  1. Miscible with paraffin
  2. High refraction index
  3. Makes tissue transparent due to their RFI
49
Q

Refractive Index (RFI)

50
Q

Clearing Agent for double embedding technique

A

Methyl Benzoate and Salicylate

51
Q

Clearing Agent for CNS specimen

A

Cedarwood Oil

52
Q

Clearing Agent for fine and delicate specimen

A

Aniline oil

53
Q

Melting point of paraffin

A

56 deg Celsius

54
Q

Temp of paraffin oven

A

+ 2-5 deg Celsius of 56 deg Celsius

55
Q

Temp of Flotation or Water Bath

A

(-) 6-10 deg Celsius of 56 deg Celsius

56
Q

Another name for Carbowax and char

A

Water soluble wax - composed of polyethylene glycol (no dehydration and clearing)

57
Q

Purified nitrocellulose (carbohydrate) impregnating media

A

Celloidin - for specimen with large hollow cavity

58
Q

Shape for sectioning

A

Truncated pyramid or 4-sided prism

59
Q

Disposable embedding molds (3)

A

Paper boats
Peel Away
Plastic Ice Trays

60
Q

Rocking Microtome is also known as

And proponent

A

Cambridge Microtome

Paldwell Trefall

61
Q

Rotary Microtome is also known as

62
Q

Sliding Microtome proponent and two types

A

Adams

  • base Sledge (moving block holder)
  • Standard Sliding (knife moving so dangerous)
63
Q

Freezing Microtome proponent

64
Q

What microtome

Is used for electron microscopy?

A

Ultrathin Microtome

65
Q

Microtome for unfrozen, unfixed specimen for enzyme demonstration?

66
Q

Plane concave knife

Measurement

A

25 mm
Less concave - celloidin (sliding)
More concave - paraffin (rotary and rocking)

67
Q

Biconcave

Measurement

A

120 mm

Rotary - paraffin

68
Q

Plane Wedge Knife

Measurement

A

100 mm

Both sides are straight (for frozen sections)

69
Q

Wedge Angle

A

14-15 deg Celsius

70
Q

Angle formed between the cutting edge

A

Bevel Angle (27-32)

71
Q

Angle formed by the sides of the wedge knives

A

Wedge Angle (14-15)

72
Q

Angle formed between the cutting facet presenting to the block and the surface of the block

A

Clearance Angle (5-15)

73
Q

Removal of gross nicks

74
Q

Direction of Honing

A

Heel to Toe (Edge first)

75
Q

Hones that give the Best result

A

Belgium Yellow

76
Q

Hones that have more polishing effect

77
Q

Hones for badly nicked knives

A

Fine Carborundum

78
Q

Removal of burr

79
Q

Direction of stropping

A

Toe to heel (Edge Last)

80
Q

Purpose of honing

A

Remove irregularities from the knife

81
Q

Purpose of stropping

A

Polish and sharpen the cutting edge

82
Q

Giving color to the sections using aqueous or alcoholic dye solution

A

Direct Staining

83
Q

Action of the dye is intensified by adding another agent (mordant or accentuator)

A

Indirect staining

84
Q

Link or bridge between the tissue and the dye

85
Q

Speeds up the staining process

A

Accentuator

86
Q

Dyes used has a staining color that is different from that of the stain itself

A

Metachromatic Stain

87
Q

Dye with the same color as the stain

A

Orthochromatic Staining

88
Q

Staining is done by injecting the dye into any parts of the animal body

A

Intravital Staining

89
Q

Ex of Intravital Staining

A

Lithium
Carmine
India Ink

90
Q

Stain living cells immediately after removal from living body

A

Supravital Staining

91
Q

Ex of Supravital staining

A

Methylene blue, brilliant cresyl blue

92
Q

Carmine + aluminum chloride =

A

Best Carmine

93
Q

Vegetable dye extracted from lichens normally colorless

-treated with ammonia and exposed to air to produce blue or violet color

94
Q

Another name

For synthetic Dyes

A

Coal Tar Dyes

95
Q

Coloring Property

A

Chromophore

96
Q

Dyeing Property

A

Auxochrome

HISTOTECH (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions