Pathology Flashcards
neurons most vulnerable to hypoxic-ischemic insults
purkinje cells of cerebellum
pyramidal cell of hippocampus and neocortex (zones 3, 5, 6)
histology of caseous necrosis
fragmented cells and debris surrounded by lymphocytes and macrophages (granuloma)
what is primary systemic amyloidosis associated with
plasma cell dycrasias (MM)
what is secondary systemic amyloidosis
systemic deposition of serum amyloid A (AA)
seen in chronic inflammatory conditions (RA, IBD, familiar mediterranean fever, protracted infection, cancer)
Fever mechanism
- pyrogens (LPS) induce macrophages to release IL-1 and TNF
- increase COX in perivascular cells of hypothalamus
- increase PGE2
- increase temp set point
which bcl-2 proteins are proapoptotic
BAX
BAK
form pores in mitochondrial membrane –> release cytochrome C (inner mito) into cytoplasm –> activation of capsases
the two pathways of extrinsic (death receptor) path
- ligand receptor interactions (FasL binding to Fas [CD95] or TNF alpha bind to receptor)
- immune cell (cytotoxic T-cell release of perforin and granzyme B)
Fas-FasL interaction is necessary in
thyme medullary negative selection
What kind of necrosis is seen with distal extremity and GI tract after chronic ischemia
gangrenous
caseous necrosis is d/t
macrophages
-wall off infecting microorganism –> granular debris
How is apoptosis different from necrosis
apoptosis does not have local inflammatory reaction
area of liver most vulnerable to ischemia
area around central vein (zone III)
histology of wet gangrene
liquefactive superimposed on coagulative
liquefactive: neutrophils and cell debris seen with bacterial infection
coagulative: preserved cell structure w/no nuclei, increased eosin staining, PMNs later to clean up
what does bcl-2 do
keeps mitochondrial membrane impermeable
prevents cytochrome C from leaking
what is primary systemic amyloidosis
systemic deposition of AL amyloid derived from Ig light chain
acute phase proteins are notably induced by
IL-6
rubor (redness) and color (warmth) is mediated by
histamine PG bradykinin NO vasodilation (SM relaxation)
MC see coagulative necrosis in what organs
heart liver spleen kidneys organs that use a lot of oxygen
Aschoff bodies in acute rheumatic heart disease is an example of what kind of necrosis
fibrinoid necrosis
(type II hypersensitivity)
lymphocytes in heart muscles layers
what does fat necrosis look like on H&E stain
dark blue
-saponification of fat with Ca
familial ventricular endomyocardium deposition of amyloid causes
restrictive cardiomyopathy and arrhythmia
what kind of necrosis do you see in brain infarcts and pancreas
liquefactive
tumor (swelling) is mediated by
endothelial contraction leukotrienes C4, D4, E4 histamine serotonin increase vascular permeability and interstitial oncotic pressure
what kind of necrosis will you see in immune vascular reactions (PAN)
fibrinoid necrosis
what is familial mediterranean fever
AR
dysfunction of neutrophils
acute fever and serousal inflammation
high SAA during attacks that deposit as AA amyloid
opsonin
fixes complement and facilitates phagocytosis
measured clinically as nonspecific sign of ongoing inflammation
CRP (positive, upregulated)
definition of pseudocyst (seen in pancreas)
cavity of fluid lined by necrotic tissue
intrinsic and extrinsic apoptosis mediated by
capsases (cytosolic proteases)
Part of kidney most vulnerable to ischemia
straight segment of proximal tubule (medulla)
thick ascending limb (medulla)
GI manifestations of systemic amyloidosis
macroglossia
hepatomegaly
malabsorption from bowel thickening
histology of dry gangrene
same as coagulative
- preserved cell architecture
- nuclei disappear
- increase cytoplasmic binding of eosin stain
- neutrophils will come in to clean up later on
liquefactive necrosis histology
early: cellular debris and macrophages
late: cystic spaces and cavitation (brain), neutrophils and cell debris seen with bacterial infection
MC organ affected by amyloid
kidney
general characteristics of reversible cell injury
- ribosomal/polysomal detachment (decrease protein synthesis)
- plasma membrane changes (blobbing)
- nuclear changes (chromatin clumping)
- rapid loss of function (myocardial cells are non contractile after 1-2 min of ischemia)
general characteristics of irreversible cell injury
- mitochondrial damage/dysfunction
- rupture of lysosomes –> autolysis
- nuclear degradation
Intrinsic pathway regulated by what family of proteins
bcl-2
neurologic manifestations of systemic amyloidosis
neuropathy
examples of localized amyloidosis
alzheimer dz T2DM medullary thyroid cancer isolated atrial amyloidosis systemic senile (age related) amyloidosis
autoimmune lymphoproliferative syndrome is caused by
defective Fas-FasL interactions
MSK manifestations of systemic amyloidosis
carpal tunnel syndrome