Patch clamping methods

Question 1

ADVANTAGES:
- Records single receptor activity
- internal environment is maintained (no wash out),
- control of extracell. solution
DISADVANTAGES:
- agonist always present (receptor eventually desensitized),
- Vm is not known ,
- no control of intracell. content

Answer 1

cell attached patch

Question 2

ADVANTAGES:
- control of intracell/extracell environment
- record populations of receptors/ion channels
- can introduce biochem. modulators
DISADVANTAGES
- wash out required
- single receptor activity is hard to observe (poor resolution)

Answer 2

Whole cell patch

Question 3

ADVANTAGES
- control of intra/extracell. milieu (environment)
- can change extracell. control drug (agonist) concentrations
DISADVANTAGES
- washout is required
- cannot easily change intracell. messengers

Answer 3

Outside-out patch

Question 4

ADVNATAGES
- control of intra/extracel. milieu
- can introduce biochem modulators (c-AMP)
DISADVANTAGES
- cannot easily change agonist conc.

Answer 4

inside- out patch