Patch Clamp Method Flashcards
1
Q
What is the purpose of the Patch Clamp Method?
A
- monitor cell electrical activities
- record cell under controlled conditions
2
Q
How to perform the Patch Clamp Method?
A
- sharp electrode approach cell
- gentle suction forms ‘giga’ seal – see resistance change
- strong suction to have continuous intracellular fluid
3
Q
What are the 2 types of recording?
A
Current Clamp (I-clamp) – records voltage
Voltage Clamp (V-clamp) – records current
4
Q
What are the configurations of Patch Recording?
A
- Cell-attached patch recording – intracellular intact
- Whole-cell recording – continuous intracellular
- Inside-out patch – cytoplasm accessible
- Outside-out patch – extracellular accessible
5
Q
How to record channels?
A
- at fixed voltage
- random instantaneous channel openings and closings
- current amplitudes the same when 2 channels open
