Pasteurella Flashcards
unfinished. no Epizootiology and Pathogenesis onwards.
Identify the bacteria
- frequently tiny, gram negative, pleomorphic coccobacilli that takes bipolar staining
- non-motile, oxidase & catalase positive, aerobic to microaerophilic (requires small amount of O2) or facultative anaerobic
- complex group of highly adapted parasitic organisms which includes Actinobacillus & Hemophilus
Pasteurella
Pasteurella comprises at least how many species?
11
Two (2) Pasteurella sp. that are important pathogens of domestic & wild animals; constituent of shipping fever complex in cattle
Pasteurella multocida
Pasteurella haemolytica
What do you call a pathogen that cause disease in healthy individuals by itself?
Primary pathogen
What do you call a pathogen that cause disease only when host’s defenses are weakened?
Opportunistic pathogen
Type species of Pasteurella, also 1st Pasteurella identified, opportunistic invader of respiratory tract of variety of animals
Pasteurella multocida
P. multocida causes what condition in cattle and birds
Primary Septicemia
Pasteurella sp. that is both primary & opportunistic respiratory pathogen of cattle & sheep, causing septicemia & mastitis in sheep
Pasteurella haemolytica
Other Pasteurella sp. recognized by Bergey’s Manual (1984)
1.) P. aerogenes
2.) P. gallinarum
3.) P. haemolytica
4.) P. pneumotropica
5.) P. ureae
Pasteurella sp. that causes septicemia in ducks & fowl listed as species incertae sedis
Pasteurella anatipestifer
Identify the Pasteurella sp.
- tiny (~ 0.3 x 0.4-0.8 um) ovoid rods
- non-sporeforming rods
- many capsular material when freshly isolated but quickly lost
- no flagella or pili
P. multocida
On what agars do P. multocida grow very well?
blood agar & infusion agars
P. multocida’s cultural growth can be enhanced by addition of what?
serum (fastidious)
Identify the Pasteurella sp. based on these cultural characteristics on BAP
- colonies are smooth, butyrous, & convex
- 1-2 mm diameter
- mousy odor (metabolic products)
- slight greenish haze (alpha or partial)
P. multocida
P. multocida always, sometimes, or rarely grow on MacConkey?
Rarely
P. multocida
liquefy gelatin:
indole:
reduce nitrate to nitrite:
liquefy gelatin: No
indole: Positive
reduce nitrate to nitrite: No
P. multocida has how many biotypes or biovars based on sugar fermentation?
7
P. multocida strains from dogs constitute biotype characterized by what?
- cannot ferment sorbitol & mannitol
- can ferment maltose
Carter’s biotype (1976)
1.) mucoid
2.) hemorrhagic septicemic
3.) porcine
4.) canine
5.) feline
3 principal colonial variants of P. multocida on agar
1.) Mucoid colonies
2.) Smooth or fluorescent colonies
3.) Rough or blue colonies
Colonial variant of P. multocida characterized by spreading growth of large, flowing, moderately virulent for mice and (Ags & Abs) not typable by usual serologic methods
Mucoid colonies
Colonial variant of P. multocida characterized as medium sized, discrete, quite virulent for mice and typable
Smooth or fluorescent colonies
Colonial variant of P. multocida characterized as small, discrete, low in virulence for mice and auto agglutinable
Rough or blue colonies
Mucoid and smooth colonies carry specific soluble ___ associated with capsular acidic polysaccharide, basis of Robert’s (1947) and Carter’s (1955) classifications
Ags
P. multocida
The smoother the colonies, the ____ virulent
The rougher the colonies, the ____ virulent
smoother colonies: more virulent
rougher colonies: less virulent
Most prevalent capsular type among P. multocida isolates from cattle, swine, poultry, & rabbits
Type A
P. multocida are shown to carry what antigens of which there are at least 11 (Arabic numerals)?
somatic or O antigens
P. multocida are commensal of which body part of mammals?
oropharynx
