Parts of a microscope

Question 1

• where visible light is transmitted through specimen
• use compound light microscope
• multipurpose use

Answer 1

bright field microsopy

Question 2

• visible light reflected on sides of specimen
• use compound light microscope with “stop” on the condenser
• view shape, movement of cells

Answer 2

dark field microscopy

Question 3

• light patterns moving through denser regions of cell transformed into a density map
• used to visualize internal structures such as endospores, granules, organelles, and external structures called cilia

Answer 3

phase-contrast microscopy

Question 4

• two beams of light
• two prisms add contrast to colors
• colored
• 3D

Answer 4

differential interference contrast microscopy

Question 5

• fluorescent dyes attached to specific cell structures (ex. DAPI, fluorescein, texas-red, etc.)
• view internal and external structures of cells and viruses
• view interactions of cell biomolecules in 2D

Answer 5

fluorescence microscopy

Question 6

• fluorescent dyes attached to specific cell structures
• view internal and external structures of cells and viruses
• view interactions of cell biomolecules in 2D or 3D

Answer 6

confocal microscopy