Part 2

Question 1

Effective on endogenous Ag or Ag that attack from the inside like tumor, virus and intracellular pathogens.

Answer 1

Class I HLA

Question 2

Example: Adenovirus and Herpes simplex block antigen presentation making it undetected by the immune system.

Answer 2

Class I HLA

Question 3

Expressed in all nucleated cells (highest in lymphocytes and undetected in liver hepatocytes).

Answer 3

Class I HLA

Question 4

Synthesized in the endoplasmic reticulum

Answer 4

Class I HLA

Question 5

Each class I antigen is a glycoprotein dimer made up by two non-covalently linked polypeptide chains.

Answer 5

Class I HLA

Question 6

For protection against viruses and parasites

Answer 6

Class I HLA

Question 7

MHC Class I deficient diseased cells trigger and activate NK cells.

Answer 7

Class I HLA

Question 8

Nonclassical Class I antigens: E, F, G (not expressed in cell surface and do not function of antigen recognition, Grantigens is expressed in trophoblast cells during 1st trimester or pregnancy to ensure tolerance for the fetus by protecting placental tissue from NK cells.

Answer 8

Class I HLA

Question 9

HLA-B8-increased resistance to HIV infection.

Answer 9

Class I HLA

Question 10

Altered cell
Tumor cell
Virally infected cell

T cell (CD8)
TnF- Beta -
Lymphotoxin

Answer 10

МНС Class I

Question 11

can not respond to virus cell. It must be complexed with HLA

Answer 11

T-cell

Question 12

Effective on exogenous Ag (exist outside the host cell and are engulfed by phagocytosis) like bacteria.

Answer 12

Class II HLA

Question 13

Found in APCs: B- lymphocytes, monocytes ,macrophages and dendritic cells.

Answer 13

Class II HLA

Question 14

Synthesized in the endoplasmic reticulum

Answer 14

Class II HLA

Question 15

MHC Class II genes: HLA-DP,DQ and DR

Answer 15

Class II HLA

Question 16

Non-Classical 11 genes: DM, DN and DO (play a role in regulation by antigen processing).

Answer 16

Class II HLA

Question 17

HLA- A, B & C

Answer 17

Class I

Question 18

HLA-DN, DO, DP, DQ, & DR

Answer 18

Class II

Question 19

Nucleated cells

Answer 19

Class I

Question 20

B cells, macrophages, other antigen presenting cells, activated T cell

Answer 20

Class II

Question 21

To present Endogenous antigen to CYTOTOXIC T cell

Answer 21

Class I

Question 22

To present Endogenous antigen to HELPER T

Answer 22

Class II

Question 23

Target cell - Cytotoxic T cell.

Answer 23

Class I

Question 24

APCs - Helper T cell

Answer 24

Class II

Question 25

• Cytotoxic T cell directed against Class I antigens are inhibited by CD 8 tells while Class Il is by CD 4

Answer 25

Class I

Question 26

Restrict activity of T regulatory cell

Answer 26

Class II

Question 27

Importance of HLA typing:

Answer 27

Tissue / Organ Transplant
Diséase Association
Paternity Testing

Question 28

: identifies class I and II HLA Ag

Answer 28

Lymphotoxicity Testing

Question 29

a. Class I
• WB + Ficollhypaque solution - (centrifuge) - layer of plasma, mix T and B cells, Ficoll hypaque, RBC, granulocytes
• Mixture of T and B cells + antiserum (of known HLA specificity) — (tryphan blue) > Blue (+)/unstained(-)
• Use of Polyspecific Reagents:
Ab to HLA A1/A2/A3
Ab to HLA A2/A4/A6
Ab to HLA A3/A9/A8

b. Class II
• Same procedure with class I
• Mixture ofT and B cells Nylon Wool Technique
Nylon wool will absorb B cells
B cells + Anti-serum (tryphan blue) → Blue (+); Unstained (-)

Answer 29

Lymphotoxicity Testing

Question 30

Determine the compatibility of related antigens (class 11)

Answer 30

Mixed Lymphocyte Reaction

Question 31

Px Lymphocyte + Donor Lymphocyte → Incompatible (Increase Lymphocyte Proliferation of patient)
Inactivated, irradiated or treated with Mitomycin C.

Measure the increase of proliferation by 3H (tritriated hydrogen) = Increase Radioactivity (incompatible)

Scintillation Counter- measure radioactivity.

Answer 31

Mixed Lymphocyte Reaction

Question 32

Nature of Immune Response

Answer 32

nature of immunogen itself
Immunogen processing
genetic coding of MHC molecules that must combine with an immunogen before T cells are able to respond.
Immunogen presentation

Question 33

Triggers immune response

Answer 33

Immunogen

Question 34

Immunogen: macromolecules (at least 10,000 MW) that is capable of triggering an adaptive immune response from an immunocompetent host by:

Question 35

Immunogen: macromolecules (at least 10,000 MW) that is capable of triggering an adaptive immune response from an immunocompetent host by:

Question 36

Substance with the ability to react with an antibody or sensitized T cell antigen receptor but may not elicit irrimune response.

Answer 36

Antigen

Question 37

It Induces a specific response by producing a specific antibody

Answer 37

Antigen

Question 38

It Induces a specific response by producing a specific antibody

Answer 38

Antigen

Question 39

It Induces a specific response by producing a specific antibody

Answer 39

Antigen

Question 40

It Induces a specific response by producing a specific antibody

Answer 40

Antigen

Question 41

NOTE: all [?] are antigens but not all antigens are immunogens

Answer 41

immunogens

Question 42

NOTE: all [?] are antigens but not all antigens are immunogens

Answer 42

immunogens

Answer 43

Carrier

Answer 44

Carrier