Part 2

Question 1

A stock solution is serially diluted in 1:3 dilution steps. If the initial concentration is 10 M, what will be the concentration after three such dilution steps?

Answer 1

0.37037 M

Question 2

You are asked to prepare a 10-fold serial dilution series to achieve final concentration of 10^-1 M to 10^-6 from a 1 M solution. What are the final volumes if you start with 1 mL of stock solution and each dilution is performed to a final volume of 10 mL?

Answer 2

final vol: 10 mL= 9mL diluent + 1mLfrom previous concentration

Question 3

Calculate the cumulative error if the pipetting error for each step is 1% in a 5-step 1:10 serial dilution series starting from a 1 M solution.

Answer 3

0.011111 M

Question 4

If you perform a 1:2 dilution followed by a 1:4 dilution starting with a 0.8 M solution, what will be the final concentration?

Answer 4

0.1 M

Question 5

Calculate the total volume of solvent added to perform a 10-step 1:2 serial dilution starting with 1 mL of stock solution.

Answer 5

1023 mL

Question 6

How would you prepare 100 mL of a 0.005 M from a 0.5 M stock solution using serial dilution steps?

Answer 6

1mL

Question 7

A laboratory requires 20 mL of 0.01 M working solution from a 1 M stock solution using serial dilution. Describe the steps and volumes needed.

Answer 7

0.2 mL

Question 8

Combine a 1:5 dilution followed by a 1:20 dilution. If the starting concentration is 0.6 M, what is the final concentration?

Answer 8

0.006 M

Question 9

An enzyme stock solution has an activity of 200 U/mL. Prepare a series of five 1:10 serial dilutions. Calculate the enzyme activity for each dilution step.

Answer 9

200, 20, 2, 0.2, 0.02, 0.002 U/mL

Question 10

To achieve a final concentration of 0.01 M from a 1 M stock solution through a series of 1:2 dilutions, calculate the number of dilution steps required and the total volume of solvent needed if starting with 1 mL of stock solution.

Answer 10

dilution steps= 4, total volume of solvent= 15 mL