Part 1 Flashcards
The scientific study or diagnostic examination that deals with the blood serum (noncellular portion), especially with regard to the immune response to pathogens or foreign substances.
SEROLOGY
This is due to due to multiple non-covalent interactions that exhibits a lock-and-key model and reactions are reversible.
ANTIGEN-ANTIBODY REACTIONS
Refers to the number of binding sites
VALENCE
Initial force of attraction that exist between a single Fab site on an antibody molecule and a single epitopeor determinant site on the corresponding antigen.
AFFINITY
What are the four forces of attractions (weak bonds) wherein dissociation can easily occur
- Ionic bonds
- Hydrogen bonds
- Hydrophobic bond
- Van der Waals forces
Attraction between a multivalent antibody and multivalent antigen.
AVIDITY
TRUE OR FALSE: HIGHER avidity = LOWER tendency of the complex to dissociate.
TRUE
What are the four TYPES OF IMMUNOLOGIC REACTIONS.
- Primary
- Secondary
- Tertiary
- Quaternary
- Results to formation of ag-ab complex or immune complex.
- Specific recognition and combination of the antigen with the binding site of its corresponding antibody
- Best detected by immunoassays
PRIMARY IMMUNOLOGIC REACTIONS
■Conformations of the amino acid chain resulting from interchain hydrogen bonding
■Can be detected and measured more readily
■Best detected by the ff. tests:
Precipitation, Agglutination, Complement Fixation
SECONDARY IMMUNOLOGIC REACTIONS
Folding of polypeptide chains through hydrophobic and hydrogen bonds
In vivoreactions
Examples:
Phagocytosis, Opsonization, Chemotaxis
TERTIARY IMMUNOLOGIC REACTIONS
Association of polypeptide subunits to form a new protein
QUARTERNARY IMMUNOLOGIC REACTION
It uses radioactive label
RADIOIMMUNOASSAY
It uses enzyme label
ENZYME IMMUNOASSAY
It uses light emitting label
CHEMILUMINESCENT ASSAY
