PARASITOLOGY Flashcards
Symbiosis
living together of phylogenetically different organisms
Mutualism
beneficial to both organisms
Commensalism
beneficial to one organism, neutral to the other
Parasitism
beneficial to one organism, harmful to the other
Definitive Host
harbors the adult or sexual stage of a parasite
Examples of Definitive Host
- Cat - DH of T. gondii
- Mosquito (Female anopheles) - DH of Plasmodium spp.
Intermediate Host
Harbors the larval or asexual stage of a parasite
Examples of Intermediate Host
- Mosquitoes - IH of Filarial worms
- Snail - IH of ALL trematodes (schistosomes and hermaphrodites)
- Man - IH (accidental) host of Echinococcus spp. (Dog is DH)
Paratenic Host
Harbors a parasite that does not develop but remains alive and if infective to the next host. Carries or bridges the parasite to the next host.
Examples of Paratenic Host
- Large fish - PH of D. Latum
- Snake, bird - PH of G. spinigerum
Obligate Parasite
Depends entirely upon its host for existence. MOST PARASITES ARE OBLIGATE PARASITES.
Reservoir
Allows the parasite’s life cycle to continue and serves as a source of human infection
Example of Reservoir
- Pig - RH of B. coli (also considered zoonotic)
Facultative Parasite
Capable of parasitic and free-living existence
Examples of Facultative Parasite
- S. stercoralis
- FLAs (Naegleria, Acanthamoeba spp.)
Classification of parasite according to number of host required
Transitory, Permanent, Periodic
Transitory parasite
Larval stage is passed in a host while the adult is free living in only 1 host
Example of transitory parasite
S. stercoralis (INDIRECT LIFE CYCLE ONLY)
Permanent parasite
Completes its life cycle in 1 host for all the life stages
Example of permanent parasite
H. nana - the only cestode that is capable of this. Also called monoxenous or homoxenous (DIRECT LIFE CYCLE ONLY)
Periodic parasite
Requires 2 or more hosts (heteroxenous) for the larval and adult stages
Example of periodic parasite
Trematodes and cestodes (Except H. nana)
Zoonotic parasite
Primarily infects animals and may be acquired by man and causes infection in man
Examples of zoonotic parasite
- B. coli
- D. immitis
- Agents of larva migrans
Accidental parasite
Occurs in an unusual host
Examples of accidental parasites
- Echinococcus
- Agents of larva migrans (maturation is arrested at larval stage - meaning life cycle will not continue)
Erratic parasite
Occurs in an unusual organ or habitat
Example of erratic parasite
- A. lumbricoides - can cause ectopic ascariasis (it is supposed to be in the small intestine)
Spurious or coprozoic parasites
Passes through the intestinal tract without causing any disturbance (DON’T CAUSE INFECTION)
Examples of spurious or coprozoic parasite
- F. hepatica unembryonated eggs - not infectious, metacercaria is the infective stage in man
- C. hepatica unembryonated eggs
Endoparasite
Lives inside the body of a host (MOST PARASITES ARE ENDOPARASITES)
What is it called when an endoparasite invades a host?
INFECTION
Ectoparasite
Lives on body surfaces
What is it called when an endoparasite invades a host?
INFESTATION
Examples of ectoparasites
- Lice (Pediculus humanus capitis, P. h. humanus, Phthirus pubis)
- Itch mite (Sarcoptes scabei)
Intermittent parasites
Visits the host only during feeding time
Examples of intermittent parasite
- Bed bug
- Mosquito
- Flea
- Tick
- Biting fly
Arthropods are considered as?
Ectoparasite, Intermittent parasite, vectors
Biologic Vector
transmits a parasite only after the latter has completed part of its development; may also serve as a host
Mechanical or Phoretic
not essential in the parasite’s life cycle and is responsible only for transporting the parasite via appendages or external surfaces
Examples of biological vectors
Mosquitoes
Biting flies
Kissing bugs
Ticks
Parasites transmitted by mosquitoes
Plasmodium spp., W. bancrofti, B. malayi
Parasites transmitted by biting flies
Leismania spp., T. brucei, Loa loa, Oncocerca
Parasites transmitted by kissing bugs (Reduviid or Triatomine)
T. cruzi
Parasites transmitted by ticks (Ixodes)
Babesia spp.
Examples of mechanical vectors
Cockroaches
House flies
Cockroaches and house flies are:
Mechanical vectors of A. lumbricoides and other enteric parasites (stool and soil)
Portal of Entry: Mouth/oral cavity
MOT: Ingestion
Example: ________
Most intestinal parasites
Portal of Entry: Mouth/oral cavity
MOT: Oral-anal intercourse
Example: ________
G. lamblia, E. histolytica, C. parvum, D. fragilis, E. vermicularis
Portal of Entry: Skin
MOT: Larval skin penetration
Example: ________
- Hookworms
- S. stercoralis
- Schistosoma spp.
Portal of Entry: Skin
MOT: Skin inoculation (vector-borne)
Example: ________
- Plasmodium spp.
- Filariae
- Babesia spp.
- Leismania spp.
- Trypanosoma spp.
Portal of Entry: Urogenital tract
MOT: Sexual intercourse (venereal)
Example: ________
T. vaginalis
Portal of Entry: Nasal passages
MOT: Intranasal
Example: ________
FLAs (Naegleria and Acanthamoeba spp.)
Portal of Entry: Transplacental
MOT: Vertical transmission (mother to fetus)
Example: ________
- T. gondii
- T. cruzi
- Plasmodium spp.
Autoinfection can either be:
internal or external
Internal autoinfection
within the intestine
Examples of internal autoinfection
- S. stercoralis - rhabditiform transforms into filariform in the intestine
- C. philippinensis - atypical females
- C. parvum
- T. solium - reverse peristalsis of eggs (cysticercosis, can be fatal!)
External autoinfection
hand to mouth
Examples of external autoinfection
- E. vermicularis
- H. nana
Portals of exit
Anus
Mouth
Urogenital tract
Skin
Who coined the binomial system of nomenclature?
Carl Linnaeus
Genus
represents the taxon to which the species belongs; first letter is capitalized
Specific epithet
trivial name that distinguishes the species within the genus
Protozoa
unicellular eukaryotes
Phylum Sarcomastigophora
Amoebae and flagellates
Phylum Ciliophora
Ciliates (B. coli)
Phylum Apicomplexa
Sporozoans and coccidians
Similarity between Phylum Sarcomastigophora and Ciliophora
Asexual reproduction
Locomotory organelles
Characteristics of Phylum Apicomplexa
Sexual and asexual reproduction
Generally nonmotile
Other unicellular (not true protozoa) parasites include:
- Blastocystic spp. - stramenophiles
- Microsporidians - Encephalitozoon spp., enterocytozoon spp. (obligate intracellular fungi; smallest intestinal parasites (1.5 - 3 um)
- Pneumocystis jirovecii - atypical fungus
Classification of helminths
Phylum Nemathelminthes
Phylum Platyhelminthes
Phylum Nemathelminthes
Nematodes (roundworms) - non hermaphroditic
Phylum Platyhelminthes
Tramatodes (flukes) - hermaphrodites (monoecious flukes) and non-hermaphrodites (schistosomes)
Cestodes (tapeworms) - hermaphroditic
Classification of arthropods
Class Arachnida
Class Insecta
Class Crustacea
Class Arachnida
Ticks
Mites
Chiggers (bacteriology - mite larvae)
Class Insecta
Mosquitoes
Flies
Midges
Bugs
Lice
Fleas & Beetles (IH of Hymenolepsis and D. caninum)
Cockroaches (Mechanical vector of A. lumbricoides)
Class Crustacea
Crabs and crayfish (2nd IH of P. westermani)
Copepods - cyclops, Diaptomus spp. (IH of D. medinensis, G. spinigerum; 1st IH of D. latum and Spinometra)
Clearance period of antacids, antidiarrheals, barium, bismuth, laxatives (leave crystalline residues)
7-10 days
Clearance period of antimicrobial agents
2-3 weeks
Clearance period of gallbladder dyes
2 weeks
Sufficient quantity of stool specimen
2-5 grams (thumb sized) - formed
5-6 tbsp - liquid
What should the label contain in a stool specimen?
Patient’s name, age, sex, date and time of collection
Number of specimens to be examined for stool in parasitology:
3 spx collected on separate days within a 10 day period (to recover the parasites that shed diagnostic forms intermittently - e.g. G. lamblia, S. stercoralis)
Liquid stool specimens must be processed or preserved within
30 mins
Soft/semi-formed stool specimens must be processed or preserved within
1 hour
Formed stool specimens must be processed or preserved
up to 24 hours but must be refrigerated
For longer periods of preservation:
2-vial system - 1 part 10% formalin, 1 part PVA
Ratio of stool to preservative:
1 stool : 3 preservatives
10% formalin is an
All-purpose fixative
10% formalin can be used in:
WM (Wet Mount)
CT (Concentration Technique)
IA (Immunoassay)
Merthiolate-iodine-formalin can:
Fix and stain
Merthiolate-iodine-formalin can be used in:
WM (Wet Mount)
CT (Concentration Technique)
Sodium acetate-acetic acid formalin:
Can be used for permanent stains
Sodium acetate-acetic acid formalin can be used in:
WM (Wet Mount)
CT (Concentration Technique)
Permanent Staining (PS)
Schaudinn’s fluid w/o PVA contain:
Mercuric Chloride (toxic to man)
Schaudinn’s fluid w/o PVA can be used in:
Permanent Staining (PS)
PVA (SF w/ PVA) contain:
Mercuric Chloride (toxic to man)
PVA (SF w/ PVA) can be used in:
Permanent Staining (PS)
Modified PVA may be used in:
NAATs (PCR)
Modified PVA contain:
Zinc sulfate or copper sulfate
Modified PVA can be used in:
Permanent Staining (PS)