Parasite Examination Flashcards

1
Q

Physical examination; based on signs
and symptoms of the patient/s exhibited.

A

Clinical Diagnosis

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2
Q

Based significantly on laboratory reports
or test results

A

Laboratory Diagnosis

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3
Q
  • Important procedure in evaluating a
    disease by providing evidence of a new or
    unsuspected etiologic agent
  • Able to confirm a clinical impression that
    the condition has a parasitic nature

-Aids the physician with the appropriate
medication and helps in monitoring the
effect of a treatment regimen.

A

Laboratory Diagnosis

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4
Q

Successful laboratory identification of
parasites requires the knowledge and
practice of laboratory testing in the

A

pre-analytic, analytic, and post-analytic
steps.

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5
Q

What happens in Pre-analytic testing

A
  • Specimen Collection
    -Transport
  • Labeling
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6
Q

What happens in Analytic testing

A

Specimen Processing

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7
Q

Post-analytic

A
  • Interpretation
  • Reporting of results
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8
Q

the two methods for identifying parasite infections

A

➢ Serologic test
➢ Microscopic

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9
Q

the most common procedure performed in
the area of parasitology

A

Ova & Parasites (O&P) Examination

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10
Q

Two general components associated with
routine parasitology:

A

A. Macroscopic Examination
B. Microscopic Examination

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11
Q

Typical stool collection protocol consists of…

A

three specimens, one specimen collected
every other day or a total of three
collected in 10 days.

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12
Q

In the diagnosis of amebiasis, up to ______ specimens in _____ is acceptable.

A

six specimens in 14 days is acceptable.

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13
Q

Therapy that includes anti-diarrhea,
laxatives, barium, bismuth, or mineral oil
should be collected before therapy or not
until ________

A

5 to 7 days after the completion of therapy.

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14
Q

Collection of specimens from patients who
have taken antibiotics or antimalarial
medications should be delayed for ________

A

2 weeks following therapy.

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15
Q

Specimens should be collected in a ________

A

clean, watertight container with a tight-fitting lid

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16
Q

The acceptable amount of stool required
for parasite study is ______

A

2 to 5 g, often referred to as the size of a walnut.

17
Q

Criteria for specimen rejection

A
  • Urine contamination
  • Water contamination
  • Stools retrieved from the toilet bowl
  • Toilet paper in the stool specimen
  • Specimen container has NO LABEL
18
Q

What should be done when sampling handling?

A

-Gloves and a protective coat should be
worn at all times

-Universal precautions

19
Q

What is the time frame

A

➢ Fresh specimen – to demonstrate the
motility of the protozoan trophozoites.
➢ Liquid specimens = within 30 minutes
➢ Semi-formed specimens- 1 hour .
➢ Formed stool - 24 hours

20
Q

Recommended ratio is three parts fixative
to one part stool

A

1:3 ratio

21
Q

Fixatives are substances that ______ the
morphology of protozoa

A

Preserve and prevent further development

22
Q

The specimen must be fixed in the
preservative for at least _____

A

30 mins

23
Q

Describe the purpose of Formalin what are the advantages and disadvantages?

A

● An all-purpose fixative for the recovery of
protozoa and helminths.
● 5% concentration ideally preserves
protozoan cysts
● 10% concentration preserves helminth
eggs and larvae.

Advantages:

  • It is easy to prepare
  • It preserves specimens for up to several years

Disadvantages:

  • It does not preserve parasite morphology
    adequately for permanent smears
  • Trophozoites usually cannot be recovered
    and morphologic details of cysts and eggs
    may fade with time.
24
Q

Describe the purpose of Polyvinyl Alcohol (PVA)
what are the advantages and disadvantages?

A

● Plastic powder that acts as an adhesive
for the stool specimen when preparing
slides for staining.
● PVA is most often combined with
Schaudinn solution, which usually
contains zinc sulfate, copper sulfate, or
mercuric chloride.

Advantage:

● The greatest advantage of this
preservative is that it can be used for the
preparation of a permanent stained smear.

Disadvantages:

● The biggest disadvantage of the use of
PVA is that the Schaudinn solution
contains mercuric chloride.
● The recovery of certain parasites is not as
effective as when formalin is used.

25
Q

Describe the purpose of Sodium Acetate Formalin (SAF) what are the advantages and disadvantages?

A

● Alternative to the use of PVA and
Schaudinn fixative.

Advantage:

● Mercury-free

Disadvantages:

● The adhesive properties of SAF are not
good, the addition of albumin to the
microscope slide may be necessary to
ensure the adhesion of the specimen to
the slide.
● Protozoa morphology from SAF-preserved
specimens is not as clear in permanent
stains as when mercury-containing
preservatives are used.

26
Q

Describe Merthiolate-iodine Formalin (MIF

A

● Merthiolate and Iodine act as staining
components
● Formalin acts as a preservative
● Short-shelf life