Paraffin Wax Impregnation

Question 1

Simplest
Most common
Routinely used
Best embedding medium

Answer 1

Paraffin wax

Question 2

Melting point of paraffin wax used in routine work

Answer 2

56 C

Question 3

Other wax melting point

Answer 3

45
52
56
58

Question 4

Part of autotechnicon that transfers tissue to different processing reagent

Answer 4

Transfer arm

Question 5

Part of autotechnicon that facilitates removal of cover plate

Answer 5

Spring-loaded plunger

Question 6

Covers the beakers of autotechnicon

Answer 6

Cover plate

Question 7

Part of autotechnicon that controls the time needed for each processing step
Sets transfer arm and mechanism into motion

Answer 7

Electrical clock/Timing disc

Question 8

Type of autotechnicon

Answer 8

Tissue transfer/Dip & dunk

Fluid transfer/Enclosed type

Question 9

Wax impregnation under negative atmospheric pressure inside an embedding oven

Answer 9

Vacuum Embedding

Question 10

Its purpose is to hasten removal of air bubbles and clearing agents to promote rapid penetration of tissues

Answer 10

Vacuum embedding

Question 11

Clearing agents that are difficult to remove and require frequent wax changes

Answer 11

Chloroform & cedarwood oil

Question 12

Clearing agents easily removed from the tissues

Answer 12

Xylene and benzene

Question 13

Addition of this will hasten displacement of cedarwood oil with less tissue shrinkage

Answer 13

Benzene

Question 14

Overheated paraffin (above 60 degrees celsius) will cause

Answer 14

Shrinkage & hardening tissue

Complete destruction of lymphoid tissues

Question 15

Filter paper used to filter used trimmed wax before reuse

Answer 15

Green’s No. 904

Question 16

Temperature to remove water in used wax before reuse (can be used only twice)

Answer 16

100-105 degrees celsius

Question 17

Mixture of paraffin and synthetic plastic polymers

With a melting point of 56-57 degrees celsius

Answer 17

Paraplast

Question 18

Similar to paraplast

Melting point of 56-58 degrees celsius

Answer 18

Embeddol

Question 19

Semisynthetic wax for embedding eye specimen

Answer 19

Bioloid

Question 20

Product of paraffin with rubber

Answer 20

Tissue mat

Question 21

Harder than paraffin
Melting point of 46-48 degrees celsius
Requires 3-4 changes of wax
Done in heavy duty microtome

Answer 21

Ester wax

Question 22

Melting point of 38-42 degrees celsius
For enzyme histochemical studies & cytological preservation
Hygroscopic
Difficult to float out

Answer 22

Carbowax/polyethylene glycol

Question 23

Used for carbowax in order for it to float out in water bath

Answer 23

Use of soap to water or 10% polyethylene glycol 900 in water

Question 24

Use for enzyme and histochemical studies

Answer 24

PVA (polyvinyl alcohol)